Protocol for Increasing the Sensitivity of MS-Based Protein Detection in Human Chorionic Villi

Protocol for Increasing the Sensitivity of MS-Based Protein Detection in Human Chorionic Villi

An important step in the proteomic analysis of missing proteins is the use of a wide range of tissues, optimal extraction, and the processing of protein material in order to ensure the highest sensitivity in downstream protein detection. This work describes a purification protocol for identifying lo...

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Bibliographic Details
Main Authors: Timur Shkrigunov, Pavel Pogodin, Victor Zgoda, Olesya Larina, Yulia Kisrieva, Maria Klimenko, Oleg Latyshkevich, Peter Klimenko, Andrey Lisitsa, Natalia Petushkova
Format: Article
Language:English
Published: MDPI AG 2022-05-01
Series:Current Issues in Molecular Biology
Subjects:
chorionic villi
SDS extracts
1DE-gel concentration
LC–MS/MS
low-abundance proteins
elective abortion
Online Access:https://www.mdpi.com/1467-3045/44/5/140

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https://www.mdpi.com/1467-3045/44/5/140

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