A simple, economical and reproducible protein extraction protocol for proteomics studies of soybean roots

A simple, economical and reproducible protein extraction protocol for proteomics studies of soybean roots

Sample preparation is a critical step in two-dimensional gel electrophoresis (2-DE) of plant tissues. Here we describe a phenol/SDS procedure that, although greatly simplified, produced well-resolved and reproducible 2-DE profiles of protein extracts from soybean [Glycine max (L.) Merril] roots. Ext...

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Bibliographic Details
Main Authors: Elisete Pains Rodrigues, Adalgisa Ribeiro Torres, Jesiane Stefânia da Silva Batista, Luciano Huergo, Mariangela Hungria
Format: Article
Language:English
Published: Sociedade Brasileira de Genética 2012-01-01
Series:Genetics and Molecular Biology
Subjects:
2-DE
protein extraction
proteomics
soybean roots
symbiosis
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000200016

Internet

http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000200016

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