Radiation-induced changes in energy metabolism result in mitochondrial dysfunction in salivary glands
Abstract Salivary glands are indirectly damaged during radiotherapy for head and neck cancer, resulting in acute and chronic hyposalivation. Current treatments for radiation-induced hyposalivation do not permanently restore function to the gland; therefore, more mechanistic understanding of the dama...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2024-01-01
|
| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-50877-9 |
| _version_ | 1797355843052634112 |
|---|---|
| author | Lauren G. Buss Brenna A. Rheinheimer Kirsten H. Limesand |
| author_facet | Lauren G. Buss Brenna A. Rheinheimer Kirsten H. Limesand |
| author_sort | Lauren G. Buss |
| collection | DOAJ |
| description | Abstract Salivary glands are indirectly damaged during radiotherapy for head and neck cancer, resulting in acute and chronic hyposalivation. Current treatments for radiation-induced hyposalivation do not permanently restore function to the gland; therefore, more mechanistic understanding of the damage response is needed to identify therapeutic targets for lasting restoration. Energy metabolism reprogramming has been observed in cancer and wound healing models to provide necessary fuel for cell proliferation; however, there is limited understanding of alterations in energy metabolism reprogramming in tissues that fail to heal. We measured extracellular acidification and oxygen consumption rates, assessed mitochondrial DNA copy number, and tested fuel dependency of irradiated primary salivary acinar cells. Radiation treatment leads to increases in glycolytic flux, oxidative phosphorylation, and ATP production rate at acute and intermediate time points. In contrast, at chronic radiation time points there is a significant decrease in glycolytic flux, oxidative phosphorylation, and ATP production rate. Irradiated salivary glands exhibit significant decreases in spare respiratory capacity and increases in mitochondrial DNA copy number at days 5 and 30 post-treatment, suggesting a mitochondrial dysfunction phenotype. These results elucidate kinetic changes in energy metabolism reprogramming of irradiated salivary glands that may underscore the chronic loss of function phenotype. |
| first_indexed | 2024-03-08T14:17:00Z |
| format | Article |
| id | doaj.art-3b6bf634877f411fbfa1fe45da06e88c |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-03-08T14:17:00Z |
| publishDate | 2024-01-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-3b6bf634877f411fbfa1fe45da06e88c2024-01-14T12:18:05ZengNature PortfolioScientific Reports2045-23222024-01-0114111510.1038/s41598-023-50877-9Radiation-induced changes in energy metabolism result in mitochondrial dysfunction in salivary glandsLauren G. Buss0Brenna A. Rheinheimer1Kirsten H. Limesand2School of Nutritional Sciences and Wellness, University of ArizonaSchool of Nutritional Sciences and Wellness, University of ArizonaSchool of Nutritional Sciences and Wellness, University of ArizonaAbstract Salivary glands are indirectly damaged during radiotherapy for head and neck cancer, resulting in acute and chronic hyposalivation. Current treatments for radiation-induced hyposalivation do not permanently restore function to the gland; therefore, more mechanistic understanding of the damage response is needed to identify therapeutic targets for lasting restoration. Energy metabolism reprogramming has been observed in cancer and wound healing models to provide necessary fuel for cell proliferation; however, there is limited understanding of alterations in energy metabolism reprogramming in tissues that fail to heal. We measured extracellular acidification and oxygen consumption rates, assessed mitochondrial DNA copy number, and tested fuel dependency of irradiated primary salivary acinar cells. Radiation treatment leads to increases in glycolytic flux, oxidative phosphorylation, and ATP production rate at acute and intermediate time points. In contrast, at chronic radiation time points there is a significant decrease in glycolytic flux, oxidative phosphorylation, and ATP production rate. Irradiated salivary glands exhibit significant decreases in spare respiratory capacity and increases in mitochondrial DNA copy number at days 5 and 30 post-treatment, suggesting a mitochondrial dysfunction phenotype. These results elucidate kinetic changes in energy metabolism reprogramming of irradiated salivary glands that may underscore the chronic loss of function phenotype.https://doi.org/10.1038/s41598-023-50877-9 |
| spellingShingle | Lauren G. Buss Brenna A. Rheinheimer Kirsten H. Limesand Radiation-induced changes in energy metabolism result in mitochondrial dysfunction in salivary glands Scientific Reports |
| title | Radiation-induced changes in energy metabolism result in mitochondrial dysfunction in salivary glands |
| title_full | Radiation-induced changes in energy metabolism result in mitochondrial dysfunction in salivary glands |
| title_fullStr | Radiation-induced changes in energy metabolism result in mitochondrial dysfunction in salivary glands |
| title_full_unstemmed | Radiation-induced changes in energy metabolism result in mitochondrial dysfunction in salivary glands |
| title_short | Radiation-induced changes in energy metabolism result in mitochondrial dysfunction in salivary glands |
| title_sort | radiation induced changes in energy metabolism result in mitochondrial dysfunction in salivary glands |
| url | https://doi.org/10.1038/s41598-023-50877-9 |
| work_keys_str_mv | AT laurengbuss radiationinducedchangesinenergymetabolismresultinmitochondrialdysfunctioninsalivaryglands AT brennaarheinheimer radiationinducedchangesinenergymetabolismresultinmitochondrialdysfunctioninsalivaryglands AT kirstenhlimesand radiationinducedchangesinenergymetabolismresultinmitochondrialdysfunctioninsalivaryglands |