A NitroPure Nitrocellulose Membrane-Based Grapevine Virus Sampling Kit: Development and Deployment to Survey Japanese Vineyards and Nurseries

A NitroPure Nitrocellulose Membrane-Based Grapevine Virus Sampling Kit: Development and Deployment to Survey Japanese Vineyards and Nurseries

We developed a NitroPure Nitrocellulose (NPN) membrane-based method for sampling and storing grapevine sap for grapevine virus detection. We devised an efficient nucleic acid extraction method for the NPN membrane, resulting in 100% amplification success for grapevine leafroll-associated virus 2 (GL...

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Bibliographic Details
Main Authors: Mizuho Nita, Taylor Jones, Diana McHenry, Elizabeth Bush, Charlotte Oliver, Akira Kawaguchi, Akiko Nita, Miyuki Katori
Format: Article
Language:English
Published: MDPI AG 2023-10-01
Series:Viruses
Subjects:
GLRaV2
GLRaV3
GRBV
NitroPure nitrocellulose
grapevine viruses
Online Access:https://www.mdpi.com/1999-4915/15/10/2102

Internet

https://www.mdpi.com/1999-4915/15/10/2102

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