A NitroPure Nitrocellulose Membrane-Based Grapevine Virus Sampling Kit: Development and Deployment to Survey Japanese Vineyards and Nurseries
We developed a NitroPure Nitrocellulose (NPN) membrane-based method for sampling and storing grapevine sap for grapevine virus detection. We devised an efficient nucleic acid extraction method for the NPN membrane, resulting in 100% amplification success for grapevine leafroll-associated virus 2 (GL...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-10-01
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| Series: | Viruses |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1999-4915/15/10/2102 |
| _version_ | 1797572017807949824 |
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| author | Mizuho Nita Taylor Jones Diana McHenry Elizabeth Bush Charlotte Oliver Akira Kawaguchi Akiko Nita Miyuki Katori |
| author_facet | Mizuho Nita Taylor Jones Diana McHenry Elizabeth Bush Charlotte Oliver Akira Kawaguchi Akiko Nita Miyuki Katori |
| author_sort | Mizuho Nita |
| collection | DOAJ |
| description | We developed a NitroPure Nitrocellulose (NPN) membrane-based method for sampling and storing grapevine sap for grapevine virus detection. We devised an efficient nucleic acid extraction method for the NPN membrane, resulting in 100% amplification success for grapevine leafroll-associated virus 2 (GLRaV2) and 3 (GLRaV3), grapevine rupestris stem pitting-associated virus (GRSPaV), grapevine virus A, grapevine virus B, and grapevine red blotch virus (GRBV). This method also allowed the storage of recoverable nucleic acid for 18 months at room temperature. We created a sampling kit to survey GLRaV2, GLRaV3, and GRBV in Japanese vineyards. We tested the kits in the field in 2018 and then conducted mail-in surveys in 2020–2021. The results showed a substantial prevalence of GLRaV3, with 48.5% of 132 sampled vines being positive. On the other hand, only 3% of samples tested positive for GLRaV2 and none for GRBV. |
| first_indexed | 2024-03-10T20:49:43Z |
| format | Article |
| id | doaj.art-3b6c24640bed4135a181c137191bc0d9 |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-10T20:49:43Z |
| publishDate | 2023-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-3b6c24640bed4135a181c137191bc0d92023-11-19T18:28:19ZengMDPI AGViruses1999-49152023-10-011510210210.3390/v15102102A NitroPure Nitrocellulose Membrane-Based Grapevine Virus Sampling Kit: Development and Deployment to Survey Japanese Vineyards and NurseriesMizuho Nita0Taylor Jones1Diana McHenry2Elizabeth Bush3Charlotte Oliver4Akira Kawaguchi5Akiko Nita6Miyuki Katori7Alson H. Smith Jr. Agricultural Research and Extension Center, Virginia Polytechnic Institute and State University (Virginia Tech), Winchester, VA 22602, USAAlson H. Smith Jr. Agricultural Research and Extension Center, Virginia Polytechnic Institute and State University (Virginia Tech), Winchester, VA 22602, USAAlson H. Smith Jr. Agricultural Research and Extension Center, Virginia Polytechnic Institute and State University (Virginia Tech), Winchester, VA 22602, USAAlson H. Smith Jr. Agricultural Research and Extension Center, Virginia Polytechnic Institute and State University (Virginia Tech), Winchester, VA 22602, USAAlson H. Smith Jr. Agricultural Research and Extension Center, Virginia Polytechnic Institute and State University (Virginia Tech), Winchester, VA 22602, USANational Agriculture and Food Research Organization (NARO), Western Region Agricultural Research Center, Hiroshima 721-8514, JapanAlson H. Smith Jr. Agricultural Research and Extension Center, Virginia Polytechnic Institute and State University (Virginia Tech), Winchester, VA 22602, USADepartment of Law and Economics, Shinshu University, Nagano 390-8621, JapanWe developed a NitroPure Nitrocellulose (NPN) membrane-based method for sampling and storing grapevine sap for grapevine virus detection. We devised an efficient nucleic acid extraction method for the NPN membrane, resulting in 100% amplification success for grapevine leafroll-associated virus 2 (GLRaV2) and 3 (GLRaV3), grapevine rupestris stem pitting-associated virus (GRSPaV), grapevine virus A, grapevine virus B, and grapevine red blotch virus (GRBV). This method also allowed the storage of recoverable nucleic acid for 18 months at room temperature. We created a sampling kit to survey GLRaV2, GLRaV3, and GRBV in Japanese vineyards. We tested the kits in the field in 2018 and then conducted mail-in surveys in 2020–2021. The results showed a substantial prevalence of GLRaV3, with 48.5% of 132 sampled vines being positive. On the other hand, only 3% of samples tested positive for GLRaV2 and none for GRBV.https://www.mdpi.com/1999-4915/15/10/2102GLRaV2GLRaV3GRBVNitroPure nitrocellulosegrapevine viruses |
| spellingShingle | Mizuho Nita Taylor Jones Diana McHenry Elizabeth Bush Charlotte Oliver Akira Kawaguchi Akiko Nita Miyuki Katori A NitroPure Nitrocellulose Membrane-Based Grapevine Virus Sampling Kit: Development and Deployment to Survey Japanese Vineyards and Nurseries Viruses GLRaV2 GLRaV3 GRBV NitroPure nitrocellulose grapevine viruses |
| title | A NitroPure Nitrocellulose Membrane-Based Grapevine Virus Sampling Kit: Development and Deployment to Survey Japanese Vineyards and Nurseries |
| title_full | A NitroPure Nitrocellulose Membrane-Based Grapevine Virus Sampling Kit: Development and Deployment to Survey Japanese Vineyards and Nurseries |
| title_fullStr | A NitroPure Nitrocellulose Membrane-Based Grapevine Virus Sampling Kit: Development and Deployment to Survey Japanese Vineyards and Nurseries |
| title_full_unstemmed | A NitroPure Nitrocellulose Membrane-Based Grapevine Virus Sampling Kit: Development and Deployment to Survey Japanese Vineyards and Nurseries |
| title_short | A NitroPure Nitrocellulose Membrane-Based Grapevine Virus Sampling Kit: Development and Deployment to Survey Japanese Vineyards and Nurseries |
| title_sort | nitropure nitrocellulose membrane based grapevine virus sampling kit development and deployment to survey japanese vineyards and nurseries |
| topic | GLRaV2 GLRaV3 GRBV NitroPure nitrocellulose grapevine viruses |
| url | https://www.mdpi.com/1999-4915/15/10/2102 |
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