Assessment of swim-up and discontinuous density gradient in sperm sex preselection for bovine embryo production
The purpose of this work was to associate the modified swim-up method with centrifugation in density gradient for the separation of X-bearing spermatozoa. Sperm viability and integrity were evaluated through the Trypan Blue/Giemsa staining method. Quality control of centrifuged spermatozoa was perfo...
Main Authors: | , , , , , , , , , , |
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Format: | Article |
Language: | English |
Published: |
Universidade Federal de Minas Gerais
2012-06-01
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Series: | Arquivo Brasileiro de Medicina Veterinária e Zootecnia |
Subjects: | |
Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352012000300001&lng=en&tlng=en |