Assessment of swim-up and discontinuous density gradient in sperm sex preselection for bovine embryo production

The purpose of this work was to associate the modified swim-up method with centrifugation in density gradient for the separation of X-bearing spermatozoa. Sperm viability and integrity were evaluated through the Trypan Blue/Giemsa staining method. Quality control of centrifuged spermatozoa was perfo...

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Bibliographic Details
Main Authors: A.C Lucio, M.V Resende, J.A. Dernowseck-Meirelles, A.P. Perini, L.Z Oliveira, M.C.V Miguel, A.S Carmo, S.Y Tomita, B.C.A Alves, F.A.T. Fazano, V.F.M.H. Lima
Format: Article
Language:English
Published: Universidade Federal de Minas Gerais 2012-06-01
Series:Arquivo Brasileiro de Medicina Veterinária e Zootecnia
Subjects:
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352012000300001&lng=en&tlng=en