Evaluation of Safety and Probiotic Potential of <i>Enterococcus faecalis</i> MG5206 and <i>Enterococcus faecium</i> MG5232 Isolated from Kimchi, a Korean Fermented Cabbage
The purpose of this study was to evaluate the genotypic and phenotypic toxicity of <i>Enterococcus faecalis</i> MG5206 and <i>Enterococcus faecium</i> MG5232 isolated from kimchi (fermented vegetable cabbage). In this study, the genotypic toxicity of the strains MG5206 and MG...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-10-01
|
| Series: | Microorganisms |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-2607/10/10/2070 |
| _version_ | 1797471060107460608 |
|---|---|
| author | YongGyeong Kim Soo-Im Choi Yulah Jeong Chang-Ho Kang |
| author_facet | YongGyeong Kim Soo-Im Choi Yulah Jeong Chang-Ho Kang |
| author_sort | YongGyeong Kim |
| collection | DOAJ |
| description | The purpose of this study was to evaluate the genotypic and phenotypic toxicity of <i>Enterococcus faecalis</i> MG5206 and <i>Enterococcus faecium</i> MG5232 isolated from kimchi (fermented vegetable cabbage). In this study, the genotypic toxicity of the strains MG5206 and MG5232 was identified through whole-genome sequencing analysis, and phenotypic virulence, such as susceptibility to antibiotics, hemolytic activity, and gelatinase and hyaluronidase activities, was also evaluated. In addition, the in vivo toxicity of both strains was evaluated using an acute oral administration test in Sprague–Dawley rats. In all the tests, both the strains were determined to be safety by confirming that they did not show antibiotic resistance or virulence factors. In addition, these strains exhibited a low level of autoaggregation ability (37.2–66.3%) and hydrophobicity, as well as a high survival rate in gastrointestinal condition in vitro. Therefore, the safety and high gastrointestinal viability of <i>E. faecalis</i> MG5206 and <i>E. faecium </i>MG5232 suggests that both the strains could be utilized in food as potential probiotics in the future. |
| first_indexed | 2024-03-09T19:44:14Z |
| format | Article |
| id | doaj.art-3ba6ee747d1c40a0bce8d13ba5144b53 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-09T19:44:14Z |
| publishDate | 2022-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-3ba6ee747d1c40a0bce8d13ba5144b532023-11-24T01:27:55ZengMDPI AGMicroorganisms2076-26072022-10-011010207010.3390/microorganisms10102070Evaluation of Safety and Probiotic Potential of <i>Enterococcus faecalis</i> MG5206 and <i>Enterococcus faecium</i> MG5232 Isolated from Kimchi, a Korean Fermented CabbageYongGyeong Kim0Soo-Im Choi1Yulah Jeong2Chang-Ho Kang3MEDIOGEN, Co., Ltd., Biovalley 1-ro, Jecheon-si 27159, KoreaMEDIOGEN, Co., Ltd., Biovalley 1-ro, Jecheon-si 27159, KoreaMEDIOGEN, Co., Ltd., Biovalley 1-ro, Jecheon-si 27159, KoreaMEDIOGEN, Co., Ltd., Biovalley 1-ro, Jecheon-si 27159, KoreaThe purpose of this study was to evaluate the genotypic and phenotypic toxicity of <i>Enterococcus faecalis</i> MG5206 and <i>Enterococcus faecium</i> MG5232 isolated from kimchi (fermented vegetable cabbage). In this study, the genotypic toxicity of the strains MG5206 and MG5232 was identified through whole-genome sequencing analysis, and phenotypic virulence, such as susceptibility to antibiotics, hemolytic activity, and gelatinase and hyaluronidase activities, was also evaluated. In addition, the in vivo toxicity of both strains was evaluated using an acute oral administration test in Sprague–Dawley rats. In all the tests, both the strains were determined to be safety by confirming that they did not show antibiotic resistance or virulence factors. In addition, these strains exhibited a low level of autoaggregation ability (37.2–66.3%) and hydrophobicity, as well as a high survival rate in gastrointestinal condition in vitro. Therefore, the safety and high gastrointestinal viability of <i>E. faecalis</i> MG5206 and <i>E. faecium </i>MG5232 suggests that both the strains could be utilized in food as potential probiotics in the future.https://www.mdpi.com/2076-2607/10/10/2070<i>Enterococcus</i>probioticssafetytoxicity |
| spellingShingle | YongGyeong Kim Soo-Im Choi Yulah Jeong Chang-Ho Kang Evaluation of Safety and Probiotic Potential of <i>Enterococcus faecalis</i> MG5206 and <i>Enterococcus faecium</i> MG5232 Isolated from Kimchi, a Korean Fermented Cabbage Microorganisms <i>Enterococcus</i> probiotics safety toxicity |
| title | Evaluation of Safety and Probiotic Potential of <i>Enterococcus faecalis</i> MG5206 and <i>Enterococcus faecium</i> MG5232 Isolated from Kimchi, a Korean Fermented Cabbage |
| title_full | Evaluation of Safety and Probiotic Potential of <i>Enterococcus faecalis</i> MG5206 and <i>Enterococcus faecium</i> MG5232 Isolated from Kimchi, a Korean Fermented Cabbage |
| title_fullStr | Evaluation of Safety and Probiotic Potential of <i>Enterococcus faecalis</i> MG5206 and <i>Enterococcus faecium</i> MG5232 Isolated from Kimchi, a Korean Fermented Cabbage |
| title_full_unstemmed | Evaluation of Safety and Probiotic Potential of <i>Enterococcus faecalis</i> MG5206 and <i>Enterococcus faecium</i> MG5232 Isolated from Kimchi, a Korean Fermented Cabbage |
| title_short | Evaluation of Safety and Probiotic Potential of <i>Enterococcus faecalis</i> MG5206 and <i>Enterococcus faecium</i> MG5232 Isolated from Kimchi, a Korean Fermented Cabbage |
| title_sort | evaluation of safety and probiotic potential of i enterococcus faecalis i mg5206 and i enterococcus faecium i mg5232 isolated from kimchi a korean fermented cabbage |
| topic | <i>Enterococcus</i> probiotics safety toxicity |
| url | https://www.mdpi.com/2076-2607/10/10/2070 |
| work_keys_str_mv | AT yonggyeongkim evaluationofsafetyandprobioticpotentialofienterococcusfaecalisimg5206andienterococcusfaeciumimg5232isolatedfromkimchiakoreanfermentedcabbage AT sooimchoi evaluationofsafetyandprobioticpotentialofienterococcusfaecalisimg5206andienterococcusfaeciumimg5232isolatedfromkimchiakoreanfermentedcabbage AT yulahjeong evaluationofsafetyandprobioticpotentialofienterococcusfaecalisimg5206andienterococcusfaeciumimg5232isolatedfromkimchiakoreanfermentedcabbage AT changhokang evaluationofsafetyandprobioticpotentialofienterococcusfaecalisimg5206andienterococcusfaeciumimg5232isolatedfromkimchiakoreanfermentedcabbage |