Isolation of Valuable Biological Substances from Microalgae Culture
Methods for purifying, detecting, and characterizing protein concentrate, carbohydrates, lipids, and neutral fats from the microalgae were developed as a result of research. Microalgae were collected from natural sources (water, sand, soil of the Kaliningrad region, Russia). Microalgae were identifi...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-06-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/11/11/1654 |
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| author | Olga Babich Vyacheslav Dolganyuk Anna Andreeva Dmitriy Katserov Liudmila Matskova Elena Ulrikh Svetlana Ivanova Philippe Michaud Stanislav Sukhikh |
| author_facet | Olga Babich Vyacheslav Dolganyuk Anna Andreeva Dmitriy Katserov Liudmila Matskova Elena Ulrikh Svetlana Ivanova Philippe Michaud Stanislav Sukhikh |
| author_sort | Olga Babich |
| collection | DOAJ |
| description | Methods for purifying, detecting, and characterizing protein concentrate, carbohydrates, lipids, and neutral fats from the microalgae were developed as a result of research. Microalgae were collected from natural sources (water, sand, soil of the Kaliningrad region, Russia). Microalgae were identified based on morphology and polymerase chain reaction as <i>Chlorella vulgaris</i> Beijer, <i>Arthrospira platensis</i> Gomont, <i>Arthrospira platensis</i> (Nordst.) Geitl., and <i>Dunaliella salina</i> Teod. The protein content in all microalgae samples was determined using a spectrophotometer. The extracts were dried by spray freeze drying. Pressure acid hydrolysis with 1% sulfuric acid was determined to be the most effective method for extracting carbohydrates from microalgae biomass samples. The highest yield of carbohydrates (more than 56%) was obtained from <i>A. platensis</i> samples. The addition of carbohydrates to the cultivation medium increased the accumulation of fatty acids in microalgae, especially in <i>Chlorella</i>. When carbohydrates were introduced to nutrient media, neutral lipids increased by 10.9%, triacylglycerides by 10.9%, fatty acids by 13.9%, polar lipids by 3.1%, unsaponifiable substances by 13.1%, chlorophyllides by 12.1%, other impurities by 8.9% on average for all microalgae. It was demonstrated that on average the content of myristic acid increased by 10.8%, palmitic acid by 10.4%, oleic acid by 10.0%, stearic acid by 10.1%, and linoleic acid by 5.7% in all microalgae samples with the addition of carbohydrates to nutrient media. It was established that microalgae samples contained valuable components (proteins, carbohydrates, lipids, fatty acids, minerals). Thereby the study of the composition of lipids and fatty acids in microalgae, as well as the influence of carbohydrates in the nutrient medium on lipid accumulation, is a promising direction for scientific research in the fields of physiology, biochemistry, biophysics, genetics, space biology and feed additive production. |
| first_indexed | 2024-03-10T01:18:50Z |
| format | Article |
| id | doaj.art-3bc9024bf4fa4c2fbbefbb04fa6570f6 |
| institution | Directory Open Access Journal |
| issn | 2304-8158 |
| language | English |
| last_indexed | 2024-03-10T01:18:50Z |
| publishDate | 2022-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Foods |
| spelling | doaj.art-3bc9024bf4fa4c2fbbefbb04fa6570f62023-11-23T14:03:46ZengMDPI AGFoods2304-81582022-06-011111165410.3390/foods11111654Isolation of Valuable Biological Substances from Microalgae CultureOlga Babich0Vyacheslav Dolganyuk1Anna Andreeva2Dmitriy Katserov3Liudmila Matskova4Elena Ulrikh5Svetlana Ivanova6Philippe Michaud7Stanislav Sukhikh8Institute of Living Systems, Immanuel Kant Baltic Federal University, A. Nevskogo Street 14, Kaliningrad 236016, RussiaInstitute of Living Systems, Immanuel Kant Baltic Federal University, A. Nevskogo Street 14, Kaliningrad 236016, RussiaInstitute of Living Systems, Immanuel Kant Baltic Federal University, A. Nevskogo Street 14, Kaliningrad 236016, RussiaInstitute of Living Systems, Immanuel Kant Baltic Federal University, A. Nevskogo Street 14, Kaliningrad 236016, RussiaInstitute of Living Systems, Immanuel Kant Baltic Federal University, A. Nevskogo Street 14, Kaliningrad 236016, RussiaInstitute of Agroengineering and Food System, Kaliningrad State Technical University, Soviet Avenue 1, Kaliningrad 236022, RussiaNatural Nutraceutical Biotesting Laboratory, Kemerovo State University, Krasnaya Street 6, Kemerovo 650043, RussiaInstitut Pascal, Université Clermont Auvergne, CNRS, Clermont Auvergne INP, F-63000 Clermont-Ferrand, FranceInstitute of Living Systems, Immanuel Kant Baltic Federal University, A. Nevskogo Street 14, Kaliningrad 236016, RussiaMethods for purifying, detecting, and characterizing protein concentrate, carbohydrates, lipids, and neutral fats from the microalgae were developed as a result of research. Microalgae were collected from natural sources (water, sand, soil of the Kaliningrad region, Russia). Microalgae were identified based on morphology and polymerase chain reaction as <i>Chlorella vulgaris</i> Beijer, <i>Arthrospira platensis</i> Gomont, <i>Arthrospira platensis</i> (Nordst.) Geitl., and <i>Dunaliella salina</i> Teod. The protein content in all microalgae samples was determined using a spectrophotometer. The extracts were dried by spray freeze drying. Pressure acid hydrolysis with 1% sulfuric acid was determined to be the most effective method for extracting carbohydrates from microalgae biomass samples. The highest yield of carbohydrates (more than 56%) was obtained from <i>A. platensis</i> samples. The addition of carbohydrates to the cultivation medium increased the accumulation of fatty acids in microalgae, especially in <i>Chlorella</i>. When carbohydrates were introduced to nutrient media, neutral lipids increased by 10.9%, triacylglycerides by 10.9%, fatty acids by 13.9%, polar lipids by 3.1%, unsaponifiable substances by 13.1%, chlorophyllides by 12.1%, other impurities by 8.9% on average for all microalgae. It was demonstrated that on average the content of myristic acid increased by 10.8%, palmitic acid by 10.4%, oleic acid by 10.0%, stearic acid by 10.1%, and linoleic acid by 5.7% in all microalgae samples with the addition of carbohydrates to nutrient media. It was established that microalgae samples contained valuable components (proteins, carbohydrates, lipids, fatty acids, minerals). Thereby the study of the composition of lipids and fatty acids in microalgae, as well as the influence of carbohydrates in the nutrient medium on lipid accumulation, is a promising direction for scientific research in the fields of physiology, biochemistry, biophysics, genetics, space biology and feed additive production.https://www.mdpi.com/2304-8158/11/11/1654microalgaeproteinscarbohydrateslipids<i>Chlorella</i>microalgae |
| spellingShingle | Olga Babich Vyacheslav Dolganyuk Anna Andreeva Dmitriy Katserov Liudmila Matskova Elena Ulrikh Svetlana Ivanova Philippe Michaud Stanislav Sukhikh Isolation of Valuable Biological Substances from Microalgae Culture Foods microalgae proteins carbohydrates lipids <i>Chlorella</i> microalgae |
| title | Isolation of Valuable Biological Substances from Microalgae Culture |
| title_full | Isolation of Valuable Biological Substances from Microalgae Culture |
| title_fullStr | Isolation of Valuable Biological Substances from Microalgae Culture |
| title_full_unstemmed | Isolation of Valuable Biological Substances from Microalgae Culture |
| title_short | Isolation of Valuable Biological Substances from Microalgae Culture |
| title_sort | isolation of valuable biological substances from microalgae culture |
| topic | microalgae proteins carbohydrates lipids <i>Chlorella</i> microalgae |
| url | https://www.mdpi.com/2304-8158/11/11/1654 |
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