Rapid one-step real-time RT-PCR assay for the detection and quantitation of bluetongue virus
Bluetongue virus (BTV) infects domestic and wild ruminants, but it is primarily a disease of sheep. In the present study, a rapid one-step real-time RT-PCR (RT-qPCR) assay based on SYBR green chemistry was optimized by targeting the conserved region of genome segment-10 (encoding NS3). The assay was...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Indian Council of Agricultural Research
2019-04-01
|
| Series: | Indian Journal of Animal Sciences |
| Subjects: | |
| Online Access: | https://epubs.icar.org.in/index.php/IJAnS/article/view/89093 |