A transgenic toolkit for visualizing and perturbing microtubules reveals unexpected functions in the epidermis
The physiological functions of microtubules (MTs) are poorly understood in many differentiated cell types. We developed a genetic toolkit to study MT dynamics and function in diverse cells. Using TRE-EB1-GFP mice, we found that MT dynamics are strongly suppressed in differentiated keratinocytes in t...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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eLife Sciences Publications Ltd
2017-09-01
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| Series: | eLife |
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| Online Access: | https://elifesciences.org/articles/29834 |
| _version_ | 1811253172897841152 |
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| author | Andrew Muroyama Terry Lechler |
| author_facet | Andrew Muroyama Terry Lechler |
| author_sort | Andrew Muroyama |
| collection | DOAJ |
| description | The physiological functions of microtubules (MTs) are poorly understood in many differentiated cell types. We developed a genetic toolkit to study MT dynamics and function in diverse cells. Using TRE-EB1-GFP mice, we found that MT dynamics are strongly suppressed in differentiated keratinocytes in two distinct steps due to alterations in both growth rate and lifetime. To understand the functions of these MT populations, we developed TRE-spastin mice to disrupt MTs in specific cell types. MT perturbation in post-mitotic keratinocytes had profound consequences on epidermal morphogenesis. We uncoupled cell-autonomous roles in cell flattening from non-cell-autonomous requirements for MTs in regulating proliferation, differentiation, and tissue architecture. This work uncovers physiological roles for MTs in epidermal development, and the tools described here will be broadly useful to study MT dynamics and functions in mammals. |
| first_indexed | 2024-04-12T16:46:54Z |
| format | Article |
| id | doaj.art-3be6643c033b4561ad97d19ca5b670a0 |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-12T16:46:54Z |
| publishDate | 2017-09-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-3be6643c033b4561ad97d19ca5b670a02022-12-22T03:24:33ZengeLife Sciences Publications LtdeLife2050-084X2017-09-01610.7554/eLife.29834A transgenic toolkit for visualizing and perturbing microtubules reveals unexpected functions in the epidermisAndrew Muroyama0https://orcid.org/0000-0003-0701-212XTerry Lechler1https://orcid.org/0000-0003-3901-7013Department of Dermatology, Duke University Medical Center, Durham, United States; Department of Cell Biology, Duke University Medical Center, Durham, United StatesDepartment of Dermatology, Duke University Medical Center, Durham, United States; Department of Cell Biology, Duke University Medical Center, Durham, United StatesThe physiological functions of microtubules (MTs) are poorly understood in many differentiated cell types. We developed a genetic toolkit to study MT dynamics and function in diverse cells. Using TRE-EB1-GFP mice, we found that MT dynamics are strongly suppressed in differentiated keratinocytes in two distinct steps due to alterations in both growth rate and lifetime. To understand the functions of these MT populations, we developed TRE-spastin mice to disrupt MTs in specific cell types. MT perturbation in post-mitotic keratinocytes had profound consequences on epidermal morphogenesis. We uncoupled cell-autonomous roles in cell flattening from non-cell-autonomous requirements for MTs in regulating proliferation, differentiation, and tissue architecture. This work uncovers physiological roles for MTs in epidermal development, and the tools described here will be broadly useful to study MT dynamics and functions in mammals.https://elifesciences.org/articles/29834spastinmicrotubuleepidermisskinnon-centrosomaldifferentiation |
| spellingShingle | Andrew Muroyama Terry Lechler A transgenic toolkit for visualizing and perturbing microtubules reveals unexpected functions in the epidermis eLife spastin microtubule epidermis skin non-centrosomal differentiation |
| title | A transgenic toolkit for visualizing and perturbing microtubules reveals unexpected functions in the epidermis |
| title_full | A transgenic toolkit for visualizing and perturbing microtubules reveals unexpected functions in the epidermis |
| title_fullStr | A transgenic toolkit for visualizing and perturbing microtubules reveals unexpected functions in the epidermis |
| title_full_unstemmed | A transgenic toolkit for visualizing and perturbing microtubules reveals unexpected functions in the epidermis |
| title_short | A transgenic toolkit for visualizing and perturbing microtubules reveals unexpected functions in the epidermis |
| title_sort | transgenic toolkit for visualizing and perturbing microtubules reveals unexpected functions in the epidermis |
| topic | spastin microtubule epidermis skin non-centrosomal differentiation |
| url | https://elifesciences.org/articles/29834 |
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