Development of a CRISPR/Cas9-mediated gene-editing method to isolate a mutant of the unicellular green alga Parachlorella kessleri strain NIES-2152 with improved lipid productivity

Development of a CRISPR/Cas9-mediated gene-editing method to isolate a mutant of the unicellular green alga Parachlorella kessleri strain NIES-2152 with improved lipid productivity

Abstract Background Previously, we isolated a mutant of Parachlorella kessleri named strain PK4 that accumulated higher concentrations of lipids than the wild-type strain. Resequencing of the PK4 genome identified mutations in three genes which may be associated with the high-lipid phenotype. The fi...

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Bibliographic Details
Main Authors: Yuki Kasai, Satsuki Takagi, Shuhei Ota, Kotaro Ishii, Tsuyoshi Takeshita, Shigeyuki Kawano, Shigeaki Harayama
Format: Article
Language:English
Published: BMC 2024-03-01
Series:Biotechnology for Biofuels and Bioproducts
Subjects:
Parachlorella kessleri
Genetic transformation
Electroporation
Genome editing
CRISPR/Cas9
Online Access:https://doi.org/10.1186/s13068-024-02484-7

Internet

https://doi.org/10.1186/s13068-024-02484-7

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