Interaction of Cucurbit[7]uril With Protease Substrates: Application to Nanosecond Time-Resolved Fluorescence Assays
We report the use of the macrocyclic host cucurbit[7]uril (CB7) as a supramolecular additive in nanosecond time-resolved fluorescence (Nano-TRF) assays for proteases to enhance the discrimination of substrates and products and, thereby, the sensitivity. A peptide substrate was labeled with 2,3-diaza...
| Asıl Yazarlar: | , |
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| Materyal Türü: | Makale |
| Dil: | English |
| Baskı/Yayın Bilgisi: |
Frontiers Media S.A.
2020-09-01
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| Seri Bilgileri: | Frontiers in Chemistry |
| Konular: | |
| Online Erişim: | https://www.frontiersin.org/article/10.3389/fchem.2020.00806/full |