Protocol to assess receptor-ligand binding in C. elegans using adapted thermal shift assays

Summary: The Caenorhabditis elegans genome encodes a greatly expanded number of nuclear hormone receptors, many of which remain orphaned. Here, we present a protocol to assess ligand-receptor binding in C. elegans using an adapted cellular thermal shift assay and isothermal dose response. We describ...

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Bibliographic Details
Main Authors: Samantha Y. Tse, Read Pukkila-Worley
Format: Article
Language:English
Published: Elsevier 2023-09-01
Series:STAR Protocols
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166723004446