Effects of biomechanical and biochemical stimuli on angio- and vasculogenesis in a complex microvasculature-on-chip
Summary: The endothelium of blood vessels is a vital organ that reacts differently to subtle changes in stiffness and mechanical forces exerted on its environment (extracellular matrix (ECM)). Upon alteration of these biomechanical cues, endothelial cells initiate signaling pathways that govern vasc...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2023-03-01
|
| Series: | iScience |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2589004223002754 |
| _version_ | 1828004376928583680 |
|---|---|
| author | Dario Ferrari Arunima Sengupta Lyong Heo Laszlo Pethö Johann Michler Thomas Geiser Vinicio A. de Jesus Perez Wolfgang M. Kuebler Soheila Zeinali Olivier T. Guenat |
| author_facet | Dario Ferrari Arunima Sengupta Lyong Heo Laszlo Pethö Johann Michler Thomas Geiser Vinicio A. de Jesus Perez Wolfgang M. Kuebler Soheila Zeinali Olivier T. Guenat |
| author_sort | Dario Ferrari |
| collection | DOAJ |
| description | Summary: The endothelium of blood vessels is a vital organ that reacts differently to subtle changes in stiffness and mechanical forces exerted on its environment (extracellular matrix (ECM)). Upon alteration of these biomechanical cues, endothelial cells initiate signaling pathways that govern vascular remodeling. The emerging organs-on-chip technologies allow the mimicking of complex microvasculature networks, identifying the combined or singular effects of these biomechanical or biochemical stimuli. Here, we present a microvasculature-on-chip model to investigate the singular effect of ECM stiffness and mechanical cyclic stretch on vascular development. Following two different approaches for vascular growth, the effect of ECM stiffness on sprouting angiogenesis and the effect of cyclic stretch on endothelial vasculogenesis are studied. Our results indicate that ECM hydrogel stiffness controls the size of the patterned vasculature and the density of sprouting angiogenesis. RNA sequencing shows that the cellular response to stretching is characterized by the upregulation of certain genes such as ANGPTL4+5, PDE1A, and PLEC. |
| first_indexed | 2024-04-10T07:13:51Z |
| format | Article |
| id | doaj.art-3c2cdd3bf6914d0980ff44688f865d7c |
| institution | Directory Open Access Journal |
| issn | 2589-0042 |
| language | English |
| last_indexed | 2024-04-10T07:13:51Z |
| publishDate | 2023-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | iScience |
| spelling | doaj.art-3c2cdd3bf6914d0980ff44688f865d7c2023-02-26T04:27:45ZengElsevieriScience2589-00422023-03-01263106198Effects of biomechanical and biochemical stimuli on angio- and vasculogenesis in a complex microvasculature-on-chipDario Ferrari0Arunima Sengupta1Lyong Heo2Laszlo Pethö3Johann Michler4Thomas Geiser5Vinicio A. de Jesus Perez6Wolfgang M. Kuebler7Soheila Zeinali8Olivier T. Guenat9Organs-on-chip Technologies Laboratory, ARTORG Center, University of Bern, Bern, SwitzerlandOrgans-on-chip Technologies Laboratory, ARTORG Center, University of Bern, Bern, SwitzerlandStanford Center for Genomics and Personalized Medicine, Palo Alto, CA, USAEmpa, Swiss Federal Laboratories for Materials Science and Technology, Laboratory for Mechanics of Materials and Nanostructures, Thun, SwitzerlandEmpa, Swiss Federal Laboratories for Materials Science and Technology, Laboratory for Mechanics of Materials and Nanostructures, Thun, SwitzerlandDepartment of Pulmonary Medicine, Inselspital, University Hospital of Bern, Bern, Switzerland; Department for BioMedical Research, University of Bern, Bern, SwitzerlandDivision of Pulmonary, Allergy, and Critical Care Medicine, Stanford University Medical Center, Stanford, CA, USAInstitute of Physiology, Charité - Universitätsmedizin Berlin, Berlin, GermanyOrgans-on-chip Technologies Laboratory, ARTORG Center, University of Bern, Bern, SwitzerlandOrgans-on-chip Technologies Laboratory, ARTORG Center, University of Bern, Bern, Switzerland; Department of Pulmonary Medicine, Inselspital, University Hospital of Bern, Bern, Switzerland; Department of General Thoracic Surgery, Inselspital, University Hospital of Bern, Bern, Switzerland; Corresponding authorSummary: The endothelium of blood vessels is a vital organ that reacts differently to subtle changes in stiffness and mechanical forces exerted on its environment (extracellular matrix (ECM)). Upon alteration of these biomechanical cues, endothelial cells initiate signaling pathways that govern vascular remodeling. The emerging organs-on-chip technologies allow the mimicking of complex microvasculature networks, identifying the combined or singular effects of these biomechanical or biochemical stimuli. Here, we present a microvasculature-on-chip model to investigate the singular effect of ECM stiffness and mechanical cyclic stretch on vascular development. Following two different approaches for vascular growth, the effect of ECM stiffness on sprouting angiogenesis and the effect of cyclic stretch on endothelial vasculogenesis are studied. Our results indicate that ECM hydrogel stiffness controls the size of the patterned vasculature and the density of sprouting angiogenesis. RNA sequencing shows that the cellular response to stretching is characterized by the upregulation of certain genes such as ANGPTL4+5, PDE1A, and PLEC.http://www.sciencedirect.com/science/article/pii/S2589004223002754Vascular anatomyBiotechnologyTranscriptomics |
| spellingShingle | Dario Ferrari Arunima Sengupta Lyong Heo Laszlo Pethö Johann Michler Thomas Geiser Vinicio A. de Jesus Perez Wolfgang M. Kuebler Soheila Zeinali Olivier T. Guenat Effects of biomechanical and biochemical stimuli on angio- and vasculogenesis in a complex microvasculature-on-chip iScience Vascular anatomy Biotechnology Transcriptomics |
| title | Effects of biomechanical and biochemical stimuli on angio- and vasculogenesis in a complex microvasculature-on-chip |
| title_full | Effects of biomechanical and biochemical stimuli on angio- and vasculogenesis in a complex microvasculature-on-chip |
| title_fullStr | Effects of biomechanical and biochemical stimuli on angio- and vasculogenesis in a complex microvasculature-on-chip |
| title_full_unstemmed | Effects of biomechanical and biochemical stimuli on angio- and vasculogenesis in a complex microvasculature-on-chip |
| title_short | Effects of biomechanical and biochemical stimuli on angio- and vasculogenesis in a complex microvasculature-on-chip |
| title_sort | effects of biomechanical and biochemical stimuli on angio and vasculogenesis in a complex microvasculature on chip |
| topic | Vascular anatomy Biotechnology Transcriptomics |
| url | http://www.sciencedirect.com/science/article/pii/S2589004223002754 |
| work_keys_str_mv | AT darioferrari effectsofbiomechanicalandbiochemicalstimulionangioandvasculogenesisinacomplexmicrovasculatureonchip AT arunimasengupta effectsofbiomechanicalandbiochemicalstimulionangioandvasculogenesisinacomplexmicrovasculatureonchip AT lyongheo effectsofbiomechanicalandbiochemicalstimulionangioandvasculogenesisinacomplexmicrovasculatureonchip AT laszlopetho effectsofbiomechanicalandbiochemicalstimulionangioandvasculogenesisinacomplexmicrovasculatureonchip AT johannmichler effectsofbiomechanicalandbiochemicalstimulionangioandvasculogenesisinacomplexmicrovasculatureonchip AT thomasgeiser effectsofbiomechanicalandbiochemicalstimulionangioandvasculogenesisinacomplexmicrovasculatureonchip AT vinicioadejesusperez effectsofbiomechanicalandbiochemicalstimulionangioandvasculogenesisinacomplexmicrovasculatureonchip AT wolfgangmkuebler effectsofbiomechanicalandbiochemicalstimulionangioandvasculogenesisinacomplexmicrovasculatureonchip AT soheilazeinali effectsofbiomechanicalandbiochemicalstimulionangioandvasculogenesisinacomplexmicrovasculatureonchip AT oliviertguenat effectsofbiomechanicalandbiochemicalstimulionangioandvasculogenesisinacomplexmicrovasculatureonchip |