Neuregulin-1 Protects Neuronal Cells Against Damage due to CoCl-Induced Hypoxia by Suppressing Hypoxia-Inducible Factor-1α and P53 in SH-SY5Y Cells
Purpose Hypoxia-mediated neurotoxicity contributes to various neurodegenerative disorders, including Alzheimer disease. Neuregulin-1 (NRG1) plays an important role in the development and plasticity of the brain. The aim of the present study was to investigate the neuroprotective effect and the regul...
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| Format: | Article |
| Language: | English |
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Korean Continence Society
2019-11-01
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| Series: | International Neurourology Journal |
| Subjects: | |
| Online Access: | http://www.einj.org/upload/pdf/inj-1938190-095.pdf |
| _version_ | 1811206279298809856 |
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| author | Seung-Yeon Yoo Ji-Young Yoo Han-Byeol Kim Tai-Kyoung Baik Jun-Ho Lee Ran-Sook Woo |
| author_facet | Seung-Yeon Yoo Ji-Young Yoo Han-Byeol Kim Tai-Kyoung Baik Jun-Ho Lee Ran-Sook Woo |
| author_sort | Seung-Yeon Yoo |
| collection | DOAJ |
| description | Purpose Hypoxia-mediated neurotoxicity contributes to various neurodegenerative disorders, including Alzheimer disease. Neuregulin-1 (NRG1) plays an important role in the development and plasticity of the brain. The aim of the present study was to investigate the neuroprotective effect and the regulating hypoxic inducible factor of NRG1 in cobalt chloride (CoCl2) induced hypoxia. Methods Hypoxia was induced in SH-SY5Y cells by CoCl2 treatment. SH-SY5Y cells were pretreated with NRG1 and then treated with CoCl2. Western blotting, immunocytochemistry, and lactate dehydrogenase (LDH) release assays were performed to examine neuroprotective properties of NRG1 in SH-SY5Y cells. Results Our data showed that CoCl2 induced cytotoxicity and changes of hypoxia-inducible factor-1α (HIF-1α) and p53 expression in SH-SY5Y cells. However, pretreatment with NRG1 inhibited CoCl2-induced accumulation of HIF-1α and p53 stability. In addition, NRG1 significantly attenuated cell death of SH-SY5Y induced by CoCl2. Conclusions NRG1 can regulate HIF-1α and p53 to protect neurons against hypoxic damage. |
| first_indexed | 2024-04-12T03:45:02Z |
| format | Article |
| id | doaj.art-3c3220e4e9a147508925f0cf4b53c789 |
| institution | Directory Open Access Journal |
| issn | 2093-4777 2093-6931 |
| language | English |
| last_indexed | 2024-04-12T03:45:02Z |
| publishDate | 2019-11-01 |
| publisher | Korean Continence Society |
| record_format | Article |
| series | International Neurourology Journal |
| spelling | doaj.art-3c3220e4e9a147508925f0cf4b53c7892022-12-22T03:49:09ZengKorean Continence SocietyInternational Neurourology Journal2093-47772093-69312019-11-0123Suppl 2S11111810.5213/inj.1938190.095816Neuregulin-1 Protects Neuronal Cells Against Damage due to CoCl-Induced Hypoxia by Suppressing Hypoxia-Inducible Factor-1α and P53 in SH-SY5Y CellsSeung-Yeon Yoo0Ji-Young Yoo1Han-Byeol Kim2Tai-Kyoung Baik3Jun-Ho Lee4Ran-Sook Woo5 Department of Anatomy and Neuroscience, Eulji University College of Medicine, Daejeon, Korea Department of Anatomy and Neuroscience, Eulji University College of Medicine, Daejeon, Korea Department of Anatomy and Neuroscience, Eulji University College of Medicine, Daejeon, Korea Department of Anatomy and Neuroscience, Eulji University College of Medicine, Daejeon, Korea Department of Emergency Medical Technology, Daejeon University, Daejeon, Korea Department of Anatomy and Neuroscience, Eulji University College of Medicine, Daejeon, KoreaPurpose Hypoxia-mediated neurotoxicity contributes to various neurodegenerative disorders, including Alzheimer disease. Neuregulin-1 (NRG1) plays an important role in the development and plasticity of the brain. The aim of the present study was to investigate the neuroprotective effect and the regulating hypoxic inducible factor of NRG1 in cobalt chloride (CoCl2) induced hypoxia. Methods Hypoxia was induced in SH-SY5Y cells by CoCl2 treatment. SH-SY5Y cells were pretreated with NRG1 and then treated with CoCl2. Western blotting, immunocytochemistry, and lactate dehydrogenase (LDH) release assays were performed to examine neuroprotective properties of NRG1 in SH-SY5Y cells. Results Our data showed that CoCl2 induced cytotoxicity and changes of hypoxia-inducible factor-1α (HIF-1α) and p53 expression in SH-SY5Y cells. However, pretreatment with NRG1 inhibited CoCl2-induced accumulation of HIF-1α and p53 stability. In addition, NRG1 significantly attenuated cell death of SH-SY5Y induced by CoCl2. Conclusions NRG1 can regulate HIF-1α and p53 to protect neurons against hypoxic damage.http://www.einj.org/upload/pdf/inj-1938190-095.pdfneuregulin-1hypoxiacoclhif-1αp53 |
| spellingShingle | Seung-Yeon Yoo Ji-Young Yoo Han-Byeol Kim Tai-Kyoung Baik Jun-Ho Lee Ran-Sook Woo Neuregulin-1 Protects Neuronal Cells Against Damage due to CoCl-Induced Hypoxia by Suppressing Hypoxia-Inducible Factor-1α and P53 in SH-SY5Y Cells International Neurourology Journal neuregulin-1 hypoxia cocl hif-1α p53 |
| title | Neuregulin-1 Protects Neuronal Cells Against Damage due to CoCl-Induced Hypoxia by Suppressing Hypoxia-Inducible Factor-1α and P53 in SH-SY5Y Cells |
| title_full | Neuregulin-1 Protects Neuronal Cells Against Damage due to CoCl-Induced Hypoxia by Suppressing Hypoxia-Inducible Factor-1α and P53 in SH-SY5Y Cells |
| title_fullStr | Neuregulin-1 Protects Neuronal Cells Against Damage due to CoCl-Induced Hypoxia by Suppressing Hypoxia-Inducible Factor-1α and P53 in SH-SY5Y Cells |
| title_full_unstemmed | Neuregulin-1 Protects Neuronal Cells Against Damage due to CoCl-Induced Hypoxia by Suppressing Hypoxia-Inducible Factor-1α and P53 in SH-SY5Y Cells |
| title_short | Neuregulin-1 Protects Neuronal Cells Against Damage due to CoCl-Induced Hypoxia by Suppressing Hypoxia-Inducible Factor-1α and P53 in SH-SY5Y Cells |
| title_sort | neuregulin 1 protects neuronal cells against damage due to cocl induced hypoxia by suppressing hypoxia inducible factor 1α and p53 in sh sy5y cells |
| topic | neuregulin-1 hypoxia cocl hif-1α p53 |
| url | http://www.einj.org/upload/pdf/inj-1938190-095.pdf |
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