Improved gRNA secondary structures allow editing of target sites resistant to CRISPR-Cas9 cleavage

Improved gRNA secondary structures allow editing of target sites resistant to CRISPR-Cas9 cleavage

Some DNA sequences are refractory to CRISPR-Cas9 cleavage, partially due to gRNA misfolding. Here the authors engineer gRNAs to prevent misfolding and further enhanced their stability by chemical modifications allowing robust genome editing regardless of target sequence.

Bibliographic Details
Main Authors: Stephan Riesenberg, Nelly Helmbrecht, Philipp Kanis, Tomislav Maricic, Svante Pääbo
Format: Article
Language:English
Published: Nature Portfolio 2022-01-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-022-28137-7

Internet

https://doi.org/10.1038/s41467-022-28137-7

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