Characterization of Argonaute nucleases from mesophilic bacteria Paenibacillus borealis and Brevibacillus laterosporus

Characterization of Argonaute nucleases from mesophilic bacteria Paenibacillus borealis and Brevibacillus laterosporus

Abstract Thermophilic Argonaute proteins (Agos) have been shown to utilize small DNA guides for cleaving complementary DNA in vitro, which shows great potential for nucleic acid detection. In this study, we explored mesophilic Agos for the detection of small molecule by cooperating with allosteric t...

Full description

Bibliographic Details
Main Authors: Huarong Dong, Fei Huang, Xiang Guo, Xiaoyi Xu, Qian Liu, Xiao Li, Yan Feng
Format: Article
Language:English
Published: SpringerOpen 2021-12-01
Series:Bioresources and Bioprocessing
Subjects:
Mesophilic Argonaute protein
Endonuclease
DNA cleavage
Allosteric transcription factors
Detection of small molecule
Online Access:https://doi.org/10.1186/s40643-021-00478-z

Internet

https://doi.org/10.1186/s40643-021-00478-z

Similar Items