Electrochemical and theoretical studies of the interaction between anticancer drug ponatinib and dsDNA
Abstract In this study, electrochemical and theoretical studies were performed to explain the interaction mechanism between ponatinib (PNT), a third generation tyrosine kinase inhibitor, and dsDNA. The electrochemical part was conducted in phosphate-buffered saline (PBS) at physiological pH of 7.4 a...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2024-01-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-024-52609-z |
| _version_ | 1797276531333005312 |
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| author | Sylwia Smarzewska Anna Ignaczak Kamila Koszelska |
| author_facet | Sylwia Smarzewska Anna Ignaczak Kamila Koszelska |
| author_sort | Sylwia Smarzewska |
| collection | DOAJ |
| description | Abstract In this study, electrochemical and theoretical studies were performed to explain the interaction mechanism between ponatinib (PNT), a third generation tyrosine kinase inhibitor, and dsDNA. The electrochemical part was conducted in phosphate-buffered saline (PBS) at physiological pH of 7.4 and in acetate buffer with a pH of 4.7, using square wave voltammetry. A boron-doped diamond electrode was used in a bulk-incubated solution. The theoretical part was investigated using computational methods, such as the semiempirical method PM7 and density functional theory (DFT). Significant differences in the electrochemical behavior of PNT in the presence of DNA confirmed the occurrence of interactions. The results obtained in the acetate buffer strongly suggested the preferential interaction of PNT with guanine residues. However, at physiological pH, it can be concluded that PNT interacts with dGua and dAdo in the dsDNA molecule. These results are consistent with outcomes from the theoretical studies, where quantum-chemical calculations showed that both electrochemically detectable nucleobases form hydrogen bonds with the drug. These bonds appeared to be stronger with guanine than with adenine. According to the computational studies, the dsDNA major groove is the energetically preferred site for the complexation of PNT. |
| first_indexed | 2024-03-07T15:29:31Z |
| format | Article |
| id | doaj.art-3c6f061dc9334f8780258573927a61fe |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-03-07T15:29:31Z |
| publishDate | 2024-01-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-3c6f061dc9334f8780258573927a61fe2024-03-05T16:28:54ZengNature PortfolioScientific Reports2045-23222024-01-0114111410.1038/s41598-024-52609-zElectrochemical and theoretical studies of the interaction between anticancer drug ponatinib and dsDNASylwia Smarzewska0Anna Ignaczak1Kamila Koszelska2Department of Inorganic and Analytical Chemistry, University of LodzDepartment of Physical Chemistry, University of LodzDepartment of Inorganic and Analytical Chemistry, University of LodzAbstract In this study, electrochemical and theoretical studies were performed to explain the interaction mechanism between ponatinib (PNT), a third generation tyrosine kinase inhibitor, and dsDNA. The electrochemical part was conducted in phosphate-buffered saline (PBS) at physiological pH of 7.4 and in acetate buffer with a pH of 4.7, using square wave voltammetry. A boron-doped diamond electrode was used in a bulk-incubated solution. The theoretical part was investigated using computational methods, such as the semiempirical method PM7 and density functional theory (DFT). Significant differences in the electrochemical behavior of PNT in the presence of DNA confirmed the occurrence of interactions. The results obtained in the acetate buffer strongly suggested the preferential interaction of PNT with guanine residues. However, at physiological pH, it can be concluded that PNT interacts with dGua and dAdo in the dsDNA molecule. These results are consistent with outcomes from the theoretical studies, where quantum-chemical calculations showed that both electrochemically detectable nucleobases form hydrogen bonds with the drug. These bonds appeared to be stronger with guanine than with adenine. According to the computational studies, the dsDNA major groove is the energetically preferred site for the complexation of PNT.https://doi.org/10.1038/s41598-024-52609-z |
| spellingShingle | Sylwia Smarzewska Anna Ignaczak Kamila Koszelska Electrochemical and theoretical studies of the interaction between anticancer drug ponatinib and dsDNA Scientific Reports |
| title | Electrochemical and theoretical studies of the interaction between anticancer drug ponatinib and dsDNA |
| title_full | Electrochemical and theoretical studies of the interaction between anticancer drug ponatinib and dsDNA |
| title_fullStr | Electrochemical and theoretical studies of the interaction between anticancer drug ponatinib and dsDNA |
| title_full_unstemmed | Electrochemical and theoretical studies of the interaction between anticancer drug ponatinib and dsDNA |
| title_short | Electrochemical and theoretical studies of the interaction between anticancer drug ponatinib and dsDNA |
| title_sort | electrochemical and theoretical studies of the interaction between anticancer drug ponatinib and dsdna |
| url | https://doi.org/10.1038/s41598-024-52609-z |
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