Identification of Minimal p53 Promoter Region Regulated by MALAT1 in Human Lung Adenocarcinoma Cells
The MALAT1 long noncoding RNA is strongly linked to cancer progression. Here we report a MALAT1 function in repressing the promoter of p53 (TP53) tumor suppressor gene. p21 and FAS, well-known p53 targets, were upregulated by MALAT1 knockdown in A549 human lung adenocarcinoma cells. We found that th...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2018-03-01
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| Series: | Frontiers in Genetics |
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| Online Access: | http://journal.frontiersin.org/article/10.3389/fgene.2017.00208/full |
| _version_ | 1818589720724111360 |
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| author | Keiko Tano Rena Onoguchi-Mizutani Fouzia Yeasmin Fumiaki Uchiumi Yutaka Suzuki Tetsushi Yada Nobuyoshi Akimitsu |
| author_facet | Keiko Tano Rena Onoguchi-Mizutani Fouzia Yeasmin Fumiaki Uchiumi Yutaka Suzuki Tetsushi Yada Nobuyoshi Akimitsu |
| author_sort | Keiko Tano |
| collection | DOAJ |
| description | The MALAT1 long noncoding RNA is strongly linked to cancer progression. Here we report a MALAT1 function in repressing the promoter of p53 (TP53) tumor suppressor gene. p21 and FAS, well-known p53 targets, were upregulated by MALAT1 knockdown in A549 human lung adenocarcinoma cells. We found that these upregulations were mediated by transcriptional activation of p53 through MALAT1 depletion. In addition, we identified a minimal MALAT1-responsive region in the P1 promoter of p53 gene. Flow cytometry analysis revealed that MALAT1-depleted cells exhibited G1 cell cycle arrest. These results suggest that MALAT1 affects the expression of p53 target genes through repressing p53 promoter activity, leading to influence the cell cycle progression. |
| first_indexed | 2024-12-16T09:45:08Z |
| format | Article |
| id | doaj.art-3c6f5b54edad4865be937833a0ba4942 |
| institution | Directory Open Access Journal |
| issn | 1664-8021 |
| language | English |
| last_indexed | 2024-12-16T09:45:08Z |
| publishDate | 2018-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Genetics |
| spelling | doaj.art-3c6f5b54edad4865be937833a0ba49422022-12-21T22:36:11ZengFrontiers Media S.A.Frontiers in Genetics1664-80212018-03-01810.3389/fgene.2017.00208300480Identification of Minimal p53 Promoter Region Regulated by MALAT1 in Human Lung Adenocarcinoma CellsKeiko Tano0Rena Onoguchi-Mizutani1Fouzia Yeasmin2Fumiaki Uchiumi3Yutaka Suzuki4Tetsushi Yada5Nobuyoshi Akimitsu6Isotope Science Center, The University of Tokyo, Tokyo, JapanIsotope Science Center, The University of Tokyo, Tokyo, JapanIsotope Science Center, The University of Tokyo, Tokyo, JapanDepartment of Gene Regulation, Faculty of Pharmaceutical Sciences, Tokyo University of Science, Noda-shi, Chiba-ken, JapanDepartment of Computational Biology, Graduate School of Frontier Sciences, The University of Tokyo, Tokyo, JapanDepartment of Bioscience and Bioinformatics, Kyushu Institute of Technology, Kitakyushu, JapanIsotope Science Center, The University of Tokyo, Tokyo, JapanThe MALAT1 long noncoding RNA is strongly linked to cancer progression. Here we report a MALAT1 function in repressing the promoter of p53 (TP53) tumor suppressor gene. p21 and FAS, well-known p53 targets, were upregulated by MALAT1 knockdown in A549 human lung adenocarcinoma cells. We found that these upregulations were mediated by transcriptional activation of p53 through MALAT1 depletion. In addition, we identified a minimal MALAT1-responsive region in the P1 promoter of p53 gene. Flow cytometry analysis revealed that MALAT1-depleted cells exhibited G1 cell cycle arrest. These results suggest that MALAT1 affects the expression of p53 target genes through repressing p53 promoter activity, leading to influence the cell cycle progression.http://journal.frontiersin.org/article/10.3389/fgene.2017.00208/fullnoncoding RNATP53transcriptiongeneticadenocarcinomapromoter regions |
| spellingShingle | Keiko Tano Rena Onoguchi-Mizutani Fouzia Yeasmin Fumiaki Uchiumi Yutaka Suzuki Tetsushi Yada Nobuyoshi Akimitsu Identification of Minimal p53 Promoter Region Regulated by MALAT1 in Human Lung Adenocarcinoma Cells Frontiers in Genetics noncoding RNA TP53 transcription genetic adenocarcinoma promoter regions |
| title | Identification of Minimal p53 Promoter Region Regulated by MALAT1 in Human Lung Adenocarcinoma Cells |
| title_full | Identification of Minimal p53 Promoter Region Regulated by MALAT1 in Human Lung Adenocarcinoma Cells |
| title_fullStr | Identification of Minimal p53 Promoter Region Regulated by MALAT1 in Human Lung Adenocarcinoma Cells |
| title_full_unstemmed | Identification of Minimal p53 Promoter Region Regulated by MALAT1 in Human Lung Adenocarcinoma Cells |
| title_short | Identification of Minimal p53 Promoter Region Regulated by MALAT1 in Human Lung Adenocarcinoma Cells |
| title_sort | identification of minimal p53 promoter region regulated by malat1 in human lung adenocarcinoma cells |
| topic | noncoding RNA TP53 transcription genetic adenocarcinoma promoter regions |
| url | http://journal.frontiersin.org/article/10.3389/fgene.2017.00208/full |
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