The Effects of Commonly Consumed Dietary Fibres on the Gut Microbiome and Its Fibre Fermentative Capacity in Adults with Inflammatory Bowel Disease in Remission
Introduction: It has been suggested that the gut microbiome of patients with inflammatory bowel disease (IBD) is unable to ferment dietary fibre. This project explored the in vitro effect of fibre fermentation on production of short-chain fatty acids (SCFA) and on microbiome composition. Methods: Fa...
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MDPI AG
2022-03-01
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| Series: | Nutrients |
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| Online Access: | https://www.mdpi.com/2072-6643/14/5/1053 |
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| author | Konstantinos Gerasimidis Ben Nichols Mhairi McGowan Vaios Svolos Rodanthi Papadopoulou Margarita Kokkorou Martina Rebull Teresita Bello Gonzalez Richard Hansen Richard Kay Russell Daniel Richard Gaya |
| author_facet | Konstantinos Gerasimidis Ben Nichols Mhairi McGowan Vaios Svolos Rodanthi Papadopoulou Margarita Kokkorou Martina Rebull Teresita Bello Gonzalez Richard Hansen Richard Kay Russell Daniel Richard Gaya |
| author_sort | Konstantinos Gerasimidis |
| collection | DOAJ |
| description | Introduction: It has been suggested that the gut microbiome of patients with inflammatory bowel disease (IBD) is unable to ferment dietary fibre. This project explored the in vitro effect of fibre fermentation on production of short-chain fatty acids (SCFA) and on microbiome composition. Methods: Faecal samples were collected from 40 adults (>16 y) with IBD (<i>n</i> = 20 with Crohn’s disease and <i>n</i> = 20 with ulcerative colitis) in clinical remission and 20 healthy controls (HC). In vitro batch culture fermentations were carried out using as substrates maize starch, apple pectin, raftilose, wheat bran, α cellulose and a mixture of these five fibres. SCFA concentration (umol/g) was quantified with gas chromatography and microbiome was profiled with 16S rRNA sequencing. Results: Fibre fermentation did not correct the baseline microbial dysbiosis or lower diversity seen in either patients with CD or UC. For all fibres, up to 51% of baseline ASVs or genera changed in abundance in HC. In patients with IBD, fermentation of fibre substrates had no effect on species or genera abundance. Production of SCFA varied among the different fibre substrates but this was not different between the two IBD groups and compared to HC after either 5 or 24 h fermentation. Conclusions: Despite extensive microbial dysbiosis, patients with IBD have a similar capacity to ferment fibre and release SCFA as HC. Fibre supplementation alone may be unlikely to restore to a healthy status the compositional shifts characteristic of the IBD microbiome. |
| first_indexed | 2024-03-09T20:26:00Z |
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| language | English |
| last_indexed | 2024-03-09T20:26:00Z |
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| series | Nutrients |
| spelling | doaj.art-3c7909bee0534470a6dd7d7da7fcf2282023-11-23T23:33:48ZengMDPI AGNutrients2072-66432022-03-01145105310.3390/nu14051053The Effects of Commonly Consumed Dietary Fibres on the Gut Microbiome and Its Fibre Fermentative Capacity in Adults with Inflammatory Bowel Disease in RemissionKonstantinos Gerasimidis0Ben Nichols1Mhairi McGowan2Vaios Svolos3Rodanthi Papadopoulou4Margarita Kokkorou5Martina Rebull6Teresita Bello Gonzalez7Richard Hansen8Richard Kay Russell9Daniel Richard Gaya10Human Nutrition, School of Medicine, College of Medical, Veterinary and Life Sciences, University of Glasgow, New Lister Building, Glasgow Royal Infirmary, Glasgow G31 2ER, UKHuman Nutrition, School of Medicine, College of Medical, Veterinary and Life Sciences, University of Glasgow, New Lister Building, Glasgow Royal Infirmary, Glasgow G31 2ER, UKHuman Nutrition, School of Medicine, College of Medical, Veterinary and Life Sciences, University of Glasgow, New Lister Building, Glasgow Royal Infirmary, Glasgow G31 2ER, UKHuman Nutrition, School of Medicine, College of Medical, Veterinary and Life Sciences, University of Glasgow, New Lister Building, Glasgow Royal Infirmary, Glasgow G31 2ER, UKHuman Nutrition, School of Medicine, College of Medical, Veterinary and Life Sciences, University of Glasgow, New Lister Building, Glasgow Royal Infirmary, Glasgow G31 2ER, UKHuman Nutrition, School of Medicine, College of Medical, Veterinary and Life Sciences, University of Glasgow, New Lister Building, Glasgow Royal Infirmary, Glasgow G31 2ER, UKHuman Nutrition, School of Medicine, College of Medical, Veterinary and Life Sciences, University of Glasgow, New Lister Building, Glasgow Royal Infirmary, Glasgow G31 2ER, UKHuman Nutrition, School of Medicine, College of Medical, Veterinary and Life Sciences, University of Glasgow, New Lister Building, Glasgow Royal Infirmary, Glasgow G31 2ER, UKDepartment of Paediatric Gastroenterology, Hepatology and Nutrition, Royal Hospital for Children, Glasgow G51 4TF, UKDepartment of Paediatric Gastroenterology, Hepatology and Nutrition, Royal Hospital for Children, Edinburgh EH9 1LF, UKGastroenterology Unit, Glasgow Royal Infirmary, Glasgow G31 2ER, UKIntroduction: It has been suggested that the gut microbiome of patients with inflammatory bowel disease (IBD) is unable to ferment dietary fibre. This project explored the in vitro effect of fibre fermentation on production of short-chain fatty acids (SCFA) and on microbiome composition. Methods: Faecal samples were collected from 40 adults (>16 y) with IBD (<i>n</i> = 20 with Crohn’s disease and <i>n</i> = 20 with ulcerative colitis) in clinical remission and 20 healthy controls (HC). In vitro batch culture fermentations were carried out using as substrates maize starch, apple pectin, raftilose, wheat bran, α cellulose and a mixture of these five fibres. SCFA concentration (umol/g) was quantified with gas chromatography and microbiome was profiled with 16S rRNA sequencing. Results: Fibre fermentation did not correct the baseline microbial dysbiosis or lower diversity seen in either patients with CD or UC. For all fibres, up to 51% of baseline ASVs or genera changed in abundance in HC. In patients with IBD, fermentation of fibre substrates had no effect on species or genera abundance. Production of SCFA varied among the different fibre substrates but this was not different between the two IBD groups and compared to HC after either 5 or 24 h fermentation. Conclusions: Despite extensive microbial dysbiosis, patients with IBD have a similar capacity to ferment fibre and release SCFA as HC. Fibre supplementation alone may be unlikely to restore to a healthy status the compositional shifts characteristic of the IBD microbiome.https://www.mdpi.com/2072-6643/14/5/1053inflammatory bowel diseasemicrobiomefibreshort-chain fatty acidsfermentation |
| spellingShingle | Konstantinos Gerasimidis Ben Nichols Mhairi McGowan Vaios Svolos Rodanthi Papadopoulou Margarita Kokkorou Martina Rebull Teresita Bello Gonzalez Richard Hansen Richard Kay Russell Daniel Richard Gaya The Effects of Commonly Consumed Dietary Fibres on the Gut Microbiome and Its Fibre Fermentative Capacity in Adults with Inflammatory Bowel Disease in Remission Nutrients inflammatory bowel disease microbiome fibre short-chain fatty acids fermentation |
| title | The Effects of Commonly Consumed Dietary Fibres on the Gut Microbiome and Its Fibre Fermentative Capacity in Adults with Inflammatory Bowel Disease in Remission |
| title_full | The Effects of Commonly Consumed Dietary Fibres on the Gut Microbiome and Its Fibre Fermentative Capacity in Adults with Inflammatory Bowel Disease in Remission |
| title_fullStr | The Effects of Commonly Consumed Dietary Fibres on the Gut Microbiome and Its Fibre Fermentative Capacity in Adults with Inflammatory Bowel Disease in Remission |
| title_full_unstemmed | The Effects of Commonly Consumed Dietary Fibres on the Gut Microbiome and Its Fibre Fermentative Capacity in Adults with Inflammatory Bowel Disease in Remission |
| title_short | The Effects of Commonly Consumed Dietary Fibres on the Gut Microbiome and Its Fibre Fermentative Capacity in Adults with Inflammatory Bowel Disease in Remission |
| title_sort | effects of commonly consumed dietary fibres on the gut microbiome and its fibre fermentative capacity in adults with inflammatory bowel disease in remission |
| topic | inflammatory bowel disease microbiome fibre short-chain fatty acids fermentation |
| url | https://www.mdpi.com/2072-6643/14/5/1053 |
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