KP177R-based visual assay integrating RPA and CRISPR/Cas12a for the detection of African swine fever virus

KP177R-based visual assay integrating RPA and CRISPR/Cas12a for the detection of African swine fever virus

IntroductionEarly detection of the virus in the environment or in infected pigs is a critical step to stop African swine fever virus (ASFV) transmission. The p22 protein encoded by ASFV KP177R gene has been shown to have no effect on viral replication and virulence and can serve as a molecular marke...

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Bibliographic Details
Main Authors: Haorui Luan, Shujuan Wang, Lin Ju, Tong Liu, Haoyue Shi, Shengqiang Ge, Shijin Jiang, Jiaqiang Wu, Jun Peng
Format: Article
Language:English
Published: Frontiers Media S.A. 2024-04-01
Series:Frontiers in Immunology
Subjects:
African swine fever virus
KP177R gene
recombinase polymerase amplification
fluorescein-labeled
biotin-labeled
CRISPR/Cas12
Online Access:https://www.frontiersin.org/articles/10.3389/fimmu.2024.1358960/full

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https://www.frontiersin.org/articles/10.3389/fimmu.2024.1358960/full

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