Combining CRISPR-Cas-mediated terminal resolution with a novel genetic workflow to achieve high-diversity adenoviral libraries

Combining CRISPR-Cas-mediated terminal resolution with a novel genetic workflow to achieve high-diversity adenoviral libraries

While recombinant adenoviruses (rAds) are widely used in both laboratory and medical gene transfer, library-based applications using this vector platform are not readily available. Recently, we developed a new method, the CRISPR-Cas9 mediated in vivo terminal resolution aiding high-efficiency rescue...

Full description

Bibliographic Details
Main Authors: Julian Fischer, Ariana Fedotova, Lena Jaki, Erwan Sallard, Anja Erhardt, Jonas Fuchs, Zsolt Ruzsics
Format: Article
Language:English
Published: Elsevier 2024-06-01
Series:Molecular Therapy: Methods & Clinical Development
Subjects:
human adenovirus
viral vectors
cloning
viral libraries
seamless mutagenesis
vector
Online Access:http://www.sciencedirect.com/science/article/pii/S2329050124000573

Internet

http://www.sciencedirect.com/science/article/pii/S2329050124000573

Similar Items