Combining CRISPR-Cas-mediated terminal resolution with a novel genetic workflow to achieve high-diversity adenoviral libraries

Combining CRISPR-Cas-mediated terminal resolution with a novel genetic workflow to achieve high-diversity adenoviral libraries

While recombinant adenoviruses (rAds) are widely used in both laboratory and medical gene transfer, library-based applications using this vector platform are not readily available. Recently, we developed a new method, the CRISPR-Cas9 mediated in vivo terminal resolution aiding high-efficiency rescue...

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Detalles Bibliográficos
Main Authors: Julian Fischer, Ariana Fedotova, Lena Jaki, Erwan Sallard, Anja Erhardt, Jonas Fuchs, Zsolt Ruzsics
Formato: Artigo
Idioma:English
Publicado: Elsevier 2024-06-01
Series:Molecular Therapy: Methods & Clinical Development
Subjects:
human adenovirus
viral vectors
cloning
viral libraries
seamless mutagenesis
vector
Acceso en liña:http://www.sciencedirect.com/science/article/pii/S2329050124000573

Internet

http://www.sciencedirect.com/science/article/pii/S2329050124000573

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