Pigs with an INS point mutation derived from zygotes electroporated with CRISPR/Cas9 and ssODN

Pigs with an INS point mutation derived from zygotes electroporated with CRISPR/Cas9 and ssODN

Just one amino acid at the carboxy-terminus of the B chain distinguishes human insulin from porcine insulin. By introducing a precise point mutation into the porcine insulin (INS) gene, we were able to generate genetically modified pigs that secreted human insulin; these pigs may be suitable donors...

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Bibliographic Details
Main Authors: Fuminori Tanihara, Maki Hirata, Zhao Namula, Lanh Thi Kim Do, Naoaki Yoshimura, Qingyi Lin, Koki Takebayashi, Tetsushi Sakuma, Takashi Yamamoto, Takeshige Otoi
Format: Article
Language:English
Published: Frontiers Media S.A. 2023-01-01
Series:Frontiers in Cell and Developmental Biology
Subjects:
CRISPR/Cas9
INS
in vitro fertilized zygotes
pig
point mutation
ssODN
Online Access:https://www.frontiersin.org/articles/10.3389/fcell.2023.884340/full

Internet

https://www.frontiersin.org/articles/10.3389/fcell.2023.884340/full

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