Serial Measurements of Apoptotic Cell Numbers Provide Better Acceptance Criterion for PBMC Quality than a Single Measurement Prior to the T Cell Assay

Serial Measurements of Apoptotic Cell Numbers Provide Better Acceptance Criterion for PBMC Quality than a Single Measurement Prior to the T Cell Assay

As soon as Peripheral Blood Mononuclear Cells (PBMC) are isolated from whole blood, some cells begin dying. The rate of apoptotic cell death is increased when PBMC are shipped, cryopreserved, or stored under suboptimal conditions. Apoptotic cells secrete cytokines that suppress inflammation while pr...

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Bibliographic Details
Main Authors: Marie Wunsch, Richard Caspell, Stefanie Kuerten, Paul V. Lehmann, Srividya Sundararaman
Format: Article
Language:English
Published: MDPI AG 2015-01-01
Series:Cells
Subjects:
T cell assay
apoptosis
acceptance
viability
ELISPOT
Online Access:http://www.mdpi.com/2073-4409/4/1/40

Internet

http://www.mdpi.com/2073-4409/4/1/40

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