Cloning and expression of meta-cleavage enzyme (CarB) of carbazole degradation pathway from Pseudomonas stutzeri

Cloning and expression of meta-cleavage enzyme (CarB) of carbazole degradation pathway from Pseudomonas stutzeri

In this work, the 1082bp PCR product corresponding to carBaBb genes that encode the heterotetrameric enzyme 2'-aminobiphenyl-2,3-diol 1,2-dioxygenase (CarB), involved in the Pseudomonas stutzeri ATCC 31258 carbazole degradation pathway, was cloned using the site-specific recombination system. R...

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Bibliographic Details
Main Authors: Ariane Leites Larentis, Tito Lívio Moitinho Alves, Orlando Bonifácio Martins
Format: Article
Language:English
Published: Instituto de Tecnologia do Paraná (Tecpar) 2005-06-01
Series:Brazilian Archives of Biology and Technology
Subjects:
Carbazole
2'-aminobiphenyl-2,3-diol dioxygenase
HOADA
heterologous gene expression
petroleum denitrogenation
biodegradation
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000400016

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http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000400016

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