Design, construction, and expression of recombinant human interferon beta gene in CHO-s cell line using EBV-based expression system

Design, construction, and expression of recombinant human interferon beta gene in CHO-s cell line using EBV-based expression system

Background and purpose: Codon optimization has been considered as a powerful strategy to increase the expression level of protein therapeutics in mammalian cells. As an empirical approach to study the effects of the codon usage and GC content on heterologous gene expression in suspension adapted Chi...

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Bibliographic Details
Main Authors: Mohadeseh Shayesteh, Fahimeh Ghasemi, Fatemeh Tabandeh, Bagher Yakhchali, Mehdi Shakibaie
Format: Article
Language:English
Published: Wolters Kluwer Medknow Publications 2020-01-01
Series:Research in Pharmaceutical Sciences
Subjects:
codon optimization; cho-s cells; ebv-based expression system; human interferon beta; recombinant protein production.
Online Access:http://www.rpsjournal.net/article.asp?issn=1735-5362;year=2020;volume=15;issue=2;spage=144;epage=153;aulast=Shayesteh

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http://www.rpsjournal.net/article.asp?issn=1735-5362;year=2020;volume=15;issue=2;spage=144;epage=153;aulast=Shayesteh

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