Screening of candidate substrates and coupling ions of transporters by thermostability shift assays

Screening of candidate substrates and coupling ions of transporters by thermostability shift assays

Substrates of most transport proteins have not been identified, limiting our understanding of their role in physiology and disease. Traditional identification methods use transport assays with radioactive compounds, but they are technically challenging and many compounds are unavailable in radioacti...

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Bibliographic Details
Main Authors: Homa Majd, Martin S King, Shane M Palmer, Anthony C Smith, Liam DH Elbourne, Ian T Paulsen, David Sharples, Peter JF Henderson, Edmund RS Kunji
Format: Article
Language:English
Published: eLife Sciences Publications Ltd 2018-10-01
Series:eLife
Subjects:
Thermothelomyces thermophila
Microbacterium liquefaciens
Tetrahymena thermophila
substrate specificity
ion coupling
thermostability
Online Access:https://elifesciences.org/articles/38821

Internet

https://elifesciences.org/articles/38821

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