Separomics applied to the proteomics and peptidomics of low-abundance proteins: choice of methods and challenges - a review

Separomics applied to the proteomics and peptidomics of low-abundance proteins: choice of methods and challenges - a review

The enrichment and isolation of proteins are considered limiting steps in proteomic studies. Identification of proteins whose expression is transient, those that are of low-abundance, and of natural peptides not described in databases, is still a great challenge. Plant extracts are in general comple...

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Bibliographic Details
Main Authors: Maria Cristina Baracat-Pereira, Meire de Oliveira Barbosa, Marcos Jorge Magalhães Júnior, Lanna Clicia Carrijo, Patrícia Dias Games, Hebréia Oliveira Almeida, José Fabiano Sena Netto, Matheus Rodrigues Pereira, Everaldo Gonçalves de Barros
Format: Article
Language:English
Published: Sociedade Brasileira de Genética 2012-01-01
Series:Genetics and Molecular Biology
Subjects:
sample preparation
complex protein extract
subproteomes
low-abundance proteins
cell wall proteins
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000200009

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http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000200009

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