Cycloolivil Isolated from <i>Nardostachys jatamansi</i> Inhibits TNF-α/IFN-γ-Induced Chemokine Production by Blocking NF-κB and JAK/STAT Activation in HaCaT Keratinocytes
<i>Nardostachys jatamansi</i> is widely used as a traditional medicine in Asian countries. Numerous recent studies have reported the biological activities of its secondary metabolites and extracts. In this study, a total of 14 components were isolated, including cycloolivil and 2-(3′-hyd...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2024-03-01
|
| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1422-0067/25/6/3342 |
| _version_ | 1797240672697188352 |
|---|---|
| author | Chi-Su Yoon Hwan Lee Zhiming Liu Linsha Dong Gyoyoung Lee Nayeon Kim Hyuncheol Oh Dong-Sung Lee |
| author_facet | Chi-Su Yoon Hwan Lee Zhiming Liu Linsha Dong Gyoyoung Lee Nayeon Kim Hyuncheol Oh Dong-Sung Lee |
| author_sort | Chi-Su Yoon |
| collection | DOAJ |
| description | <i>Nardostachys jatamansi</i> is widely used as a traditional medicine in Asian countries. Numerous recent studies have reported the biological activities of its secondary metabolites and extracts. In this study, a total of 14 components were isolated, including cycloolivil and 2-(3′-hydroxy-5′-ethoxyphenyl)-3-hydroxylmethyl-7-methoxy-2,3-dihydrobenzofuran-5-carboxylic acid, which were first discovered in <i>N. jatamansi</i>. The isolated compounds were investigated for their anti-inflammatory effects on HaCaT keratinocytes and their potential to alleviate skin inflammation. The results of the screening revealed that cycloolivil and 4β-hydroxy-8β-methoxy-10-methylene-2,9-dioxatricyclo[4.3.1.0<sup>3,7</sup>]decane reduced the production of inflammatory cytokines induced by TNF-α/IFN-γ, such as IL-6, IL-8, and RANTES, in keratinocytes. This study focused on exploring the biological effects of cycloolivil, and the results suggested that cycloolivil inhibits the expression of COX-2 proteins. Further mechanistic evaluations confirmed that the anti-inflammatory effects of cycloolivil were mediated by blockage of the NF-κB and JAK/STAT signaling pathways. These results suggest that cycloolivil isolated from <i>N. jatamansi</i> could be used to treat skin inflammatory diseases. |
| first_indexed | 2024-04-24T18:11:09Z |
| format | Article |
| id | doaj.art-3db33f9509ee4adebc4898dc0b50626a |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-04-24T18:11:09Z |
| publishDate | 2024-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-3db33f9509ee4adebc4898dc0b50626a2024-03-27T13:45:46ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672024-03-01256334210.3390/ijms25063342Cycloolivil Isolated from <i>Nardostachys jatamansi</i> Inhibits TNF-α/IFN-γ-Induced Chemokine Production by Blocking NF-κB and JAK/STAT Activation in HaCaT KeratinocytesChi-Su Yoon0Hwan Lee1Zhiming Liu2Linsha Dong3Gyoyoung Lee4Nayeon Kim5Hyuncheol Oh6Dong-Sung Lee7College of Pharmacy, Wonkwang University, Iksan 54538, Republic of KoreaResearch Institute of Pharmaceutical Sciences, College of Pharmacy, Chosun University, Gwangju 61452, Republic of KoreaResearch Institute of Pharmaceutical Sciences, College of Pharmacy, Chosun University, Gwangju 61452, Republic of KoreaResearch Institute of Pharmaceutical Sciences, College of Pharmacy, Chosun University, Gwangju 61452, Republic of KoreaResearch Institute of Pharmaceutical Sciences, College of Pharmacy, Chosun University, Gwangju 61452, Republic of KoreaResearch Institute of Pharmaceutical Sciences, College of Pharmacy, Chosun University, Gwangju 61452, Republic of KoreaCollege of Pharmacy, Wonkwang University, Iksan 54538, Republic of KoreaResearch Institute of Pharmaceutical Sciences, College of Pharmacy, Chosun University, Gwangju 61452, Republic of Korea<i>Nardostachys jatamansi</i> is widely used as a traditional medicine in Asian countries. Numerous recent studies have reported the biological activities of its secondary metabolites and extracts. In this study, a total of 14 components were isolated, including cycloolivil and 2-(3′-hydroxy-5′-ethoxyphenyl)-3-hydroxylmethyl-7-methoxy-2,3-dihydrobenzofuran-5-carboxylic acid, which were first discovered in <i>N. jatamansi</i>. The isolated compounds were investigated for their anti-inflammatory effects on HaCaT keratinocytes and their potential to alleviate skin inflammation. The results of the screening revealed that cycloolivil and 4β-hydroxy-8β-methoxy-10-methylene-2,9-dioxatricyclo[4.3.1.0<sup>3,7</sup>]decane reduced the production of inflammatory cytokines induced by TNF-α/IFN-γ, such as IL-6, IL-8, and RANTES, in keratinocytes. This study focused on exploring the biological effects of cycloolivil, and the results suggested that cycloolivil inhibits the expression of COX-2 proteins. Further mechanistic evaluations confirmed that the anti-inflammatory effects of cycloolivil were mediated by blockage of the NF-κB and JAK/STAT signaling pathways. These results suggest that cycloolivil isolated from <i>N. jatamansi</i> could be used to treat skin inflammatory diseases.https://www.mdpi.com/1422-0067/25/6/3342<i>Nardostachys jatamansi</i>anti-skin inflammationkeratinocytesJAK/STATNF-κB |
| spellingShingle | Chi-Su Yoon Hwan Lee Zhiming Liu Linsha Dong Gyoyoung Lee Nayeon Kim Hyuncheol Oh Dong-Sung Lee Cycloolivil Isolated from <i>Nardostachys jatamansi</i> Inhibits TNF-α/IFN-γ-Induced Chemokine Production by Blocking NF-κB and JAK/STAT Activation in HaCaT Keratinocytes International Journal of Molecular Sciences <i>Nardostachys jatamansi</i> anti-skin inflammation keratinocytes JAK/STAT NF-κB |
| title | Cycloolivil Isolated from <i>Nardostachys jatamansi</i> Inhibits TNF-α/IFN-γ-Induced Chemokine Production by Blocking NF-κB and JAK/STAT Activation in HaCaT Keratinocytes |
| title_full | Cycloolivil Isolated from <i>Nardostachys jatamansi</i> Inhibits TNF-α/IFN-γ-Induced Chemokine Production by Blocking NF-κB and JAK/STAT Activation in HaCaT Keratinocytes |
| title_fullStr | Cycloolivil Isolated from <i>Nardostachys jatamansi</i> Inhibits TNF-α/IFN-γ-Induced Chemokine Production by Blocking NF-κB and JAK/STAT Activation in HaCaT Keratinocytes |
| title_full_unstemmed | Cycloolivil Isolated from <i>Nardostachys jatamansi</i> Inhibits TNF-α/IFN-γ-Induced Chemokine Production by Blocking NF-κB and JAK/STAT Activation in HaCaT Keratinocytes |
| title_short | Cycloolivil Isolated from <i>Nardostachys jatamansi</i> Inhibits TNF-α/IFN-γ-Induced Chemokine Production by Blocking NF-κB and JAK/STAT Activation in HaCaT Keratinocytes |
| title_sort | cycloolivil isolated from i nardostachys jatamansi i inhibits tnf α ifn γ induced chemokine production by blocking nf κb and jak stat activation in hacat keratinocytes |
| topic | <i>Nardostachys jatamansi</i> anti-skin inflammation keratinocytes JAK/STAT NF-κB |
| url | https://www.mdpi.com/1422-0067/25/6/3342 |
| work_keys_str_mv | AT chisuyoon cycloolivilisolatedfrominardostachysjatamansiiinhibitstnfaifnginducedchemokineproductionbyblockingnfkbandjakstatactivationinhacatkeratinocytes AT hwanlee cycloolivilisolatedfrominardostachysjatamansiiinhibitstnfaifnginducedchemokineproductionbyblockingnfkbandjakstatactivationinhacatkeratinocytes AT zhimingliu cycloolivilisolatedfrominardostachysjatamansiiinhibitstnfaifnginducedchemokineproductionbyblockingnfkbandjakstatactivationinhacatkeratinocytes AT linshadong cycloolivilisolatedfrominardostachysjatamansiiinhibitstnfaifnginducedchemokineproductionbyblockingnfkbandjakstatactivationinhacatkeratinocytes AT gyoyounglee cycloolivilisolatedfrominardostachysjatamansiiinhibitstnfaifnginducedchemokineproductionbyblockingnfkbandjakstatactivationinhacatkeratinocytes AT nayeonkim cycloolivilisolatedfrominardostachysjatamansiiinhibitstnfaifnginducedchemokineproductionbyblockingnfkbandjakstatactivationinhacatkeratinocytes AT hyuncheoloh cycloolivilisolatedfrominardostachysjatamansiiinhibitstnfaifnginducedchemokineproductionbyblockingnfkbandjakstatactivationinhacatkeratinocytes AT dongsunglee cycloolivilisolatedfrominardostachysjatamansiiinhibitstnfaifnginducedchemokineproductionbyblockingnfkbandjakstatactivationinhacatkeratinocytes |