A simple negative selection method to identify adenovirus recombinants using colony PCR

A simple negative selection method to identify adenovirus recombinants using colony PCR

Background: The AdEasy system is a fast-track system for generating recombinant adenoviruses using the efficient homologous recombination machinery between shuttle and adenovirus backbone plasmids in Escherichia coli BJ5183 cells. The key step is homologous recombination in BJ5183 cells, which is dr...

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Bibliographic Details
Main Authors: Yongliang Zhao, Zongming Dong, Tengfei Li, Gang Huang
Format: Article
Language:English
Published: Elsevier 2014-01-01
Series:Electronic Journal of Biotechnology
Subjects:
Adenovirus
AdEasy
Colony PCR
Homologous recombination
RecA
Online Access:http://www.sciencedirect.com/science/article/pii/S0717345813000092

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http://www.sciencedirect.com/science/article/pii/S0717345813000092

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