Protocol for the isolation of CD8+ tumor-infiltrating lymphocytes from human tumors and their characterization by single-cell immune profiling and multiome

Protocol for the isolation of CD8+ tumor-infiltrating lymphocytes from human tumors and their characterization by single-cell immune profiling and multiome

Summary: Understanding the heterogenicity of tumor-infiltraing lymphocyte (TIL) populations and the immunobiology in human cancer is a key to establish efficient immunotherapies. Here, we have established a protocol for the characterization of CD8+ TILs in tumors by single-cell RNA-seq paired to VDJ...

Full description

Bibliographic Details
Main Authors: Carmen M. Anadon, Chaomei Zhang, Xuefeng Wang, Ling Cen, Jose R. Conejo-Garcia, Xiaoqing Yu
Format: Article
Language:English
Published: Elsevier 2022-09-01
Series:STAR Protocols
Subjects:
Bioinformatics
Sequence analysis
Cell isolation
Single cell
Flow cytometry/Mass cytometry
Clinical protocol
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166722005299

Internet

http://www.sciencedirect.com/science/article/pii/S2666166722005299

Similar Items