Vaticanol C, a resveratrol tetramer, activates PPARα and PPARβ/δ <it>in vitro </it>and <it>in vivo</it>
<p>Abstract</p> <p>Background</p> <p>Appropriate long-term drinking of red wine is associated with a reduced risk of cardiovascular disease. Resveratrol, a well-known SIRT1 activator is considered to be one of the beneficial components contained in red wine, and also de...
| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2010-05-01
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| Series: | Nutrition & Metabolism |
| Online Access: | http://www.nutritionandmetabolism.com/content/7/1/46 |
| _version_ | 1818758325493301248 |
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| author | Akao Yukihiro Iinuma Munekazu Ito Tetsuro Mishima Satoshi Katsukawa Michiko Kariya Aya Kosuge Yukiko Tamura Emi Nakata Rieko Tsukamoto Tomoko Nozawa Yoshinori Arai Yuji Namura Shobu Inoue Hiroyasu |
| author_facet | Akao Yukihiro Iinuma Munekazu Ito Tetsuro Mishima Satoshi Katsukawa Michiko Kariya Aya Kosuge Yukiko Tamura Emi Nakata Rieko Tsukamoto Tomoko Nozawa Yoshinori Arai Yuji Namura Shobu Inoue Hiroyasu |
| author_sort | Akao Yukihiro |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Appropriate long-term drinking of red wine is associated with a reduced risk of cardiovascular disease. Resveratrol, a well-known SIRT1 activator is considered to be one of the beneficial components contained in red wine, and also developed as a drug candidate. We previously demonstrated that resveratrol protects brain against ischemic stroke in mice through a PPARα-dependent mechanism. Here we report the different effects of the oligomers of resveratrol.</p> <p>Methods</p> <p>We evaluated the activation of PPARs by <it>ε</it>-viniferin, a resveratrol dimer, and vaticanol C, a resveratrol tetramer, in cell-based reporter assays using bovine arterial endothelial cells, as well as the activation of SIRT1. Moreover, we tested the metabolic action by administering vaticanol C with the high fat diet to wild-type and PPARα-knockout male mice for eight weeks.</p> <p>Results</p> <p>We show that vaticanol C activates PPARα and PPARβ/δ in cell-based reporter assays, but does not activate SIRT1. <it>ε</it>-Viniferin shows a similar radical scavenging activity as resveratrol, but neither effects on PPARs and SIRT-1. Eight-week intake of vaticanol C with a high fat diet upregulates hepatic expression of PPARα-responsive genes such as cyp4a10, cyp4a14 and FABP1, and skeletal muscle expression of PPARβ/δ-responsive genes, such as UCP3 and PDK4 (pyruvate dehydrogenase kinase, isoform 4), in wild-type, but not PPARα-knockout mice.</p> <p>Conclusion</p> <p>Vaticanol C, a resveratrol tetramer, activated PPARα and PPARβ/δ <it>in vitro </it>and <it>in vivo</it>. These findings indicate that activation of PPARα and PPARβ/δ by vaticanol C may be a novel mechanism, affording beneficial effects against lifestyle-related diseases.</p> |
| first_indexed | 2024-12-18T06:25:02Z |
| format | Article |
| id | doaj.art-3e13f504411b42afa1e9ab08c5ec0565 |
| institution | Directory Open Access Journal |
| issn | 1743-7075 |
| language | English |
| last_indexed | 2024-12-18T06:25:02Z |
| publishDate | 2010-05-01 |
| publisher | BMC |
| record_format | Article |
| series | Nutrition & Metabolism |
| spelling | doaj.art-3e13f504411b42afa1e9ab08c5ec05652022-12-21T21:18:04ZengBMCNutrition & Metabolism1743-70752010-05-01714610.1186/1743-7075-7-46Vaticanol C, a resveratrol tetramer, activates PPARα and PPARβ/δ <it>in vitro </it>and <it>in vivo</it>Akao YukihiroIinuma MunekazuIto TetsuroMishima SatoshiKatsukawa MichikoKariya AyaKosuge YukikoTamura EmiNakata RiekoTsukamoto TomokoNozawa YoshinoriArai YujiNamura ShobuInoue Hiroyasu<p>Abstract</p> <p>Background</p> <p>Appropriate long-term drinking of red wine is associated with a reduced risk of cardiovascular disease. Resveratrol, a well-known SIRT1 activator is considered to be one of the beneficial components contained in red wine, and also developed as a drug candidate. We previously demonstrated that resveratrol protects brain against ischemic stroke in mice through a PPARα-dependent mechanism. Here we report the different effects of the oligomers of resveratrol.</p> <p>Methods</p> <p>We evaluated the activation of PPARs by <it>ε</it>-viniferin, a resveratrol dimer, and vaticanol C, a resveratrol tetramer, in cell-based reporter assays using bovine arterial endothelial cells, as well as the activation of SIRT1. Moreover, we tested the metabolic action by administering vaticanol C with the high fat diet to wild-type and PPARα-knockout male mice for eight weeks.</p> <p>Results</p> <p>We show that vaticanol C activates PPARα and PPARβ/δ in cell-based reporter assays, but does not activate SIRT1. <it>ε</it>-Viniferin shows a similar radical scavenging activity as resveratrol, but neither effects on PPARs and SIRT-1. Eight-week intake of vaticanol C with a high fat diet upregulates hepatic expression of PPARα-responsive genes such as cyp4a10, cyp4a14 and FABP1, and skeletal muscle expression of PPARβ/δ-responsive genes, such as UCP3 and PDK4 (pyruvate dehydrogenase kinase, isoform 4), in wild-type, but not PPARα-knockout mice.</p> <p>Conclusion</p> <p>Vaticanol C, a resveratrol tetramer, activated PPARα and PPARβ/δ <it>in vitro </it>and <it>in vivo</it>. These findings indicate that activation of PPARα and PPARβ/δ by vaticanol C may be a novel mechanism, affording beneficial effects against lifestyle-related diseases.</p>http://www.nutritionandmetabolism.com/content/7/1/46 |
| spellingShingle | Akao Yukihiro Iinuma Munekazu Ito Tetsuro Mishima Satoshi Katsukawa Michiko Kariya Aya Kosuge Yukiko Tamura Emi Nakata Rieko Tsukamoto Tomoko Nozawa Yoshinori Arai Yuji Namura Shobu Inoue Hiroyasu Vaticanol C, a resveratrol tetramer, activates PPARα and PPARβ/δ <it>in vitro </it>and <it>in vivo</it> Nutrition & Metabolism |
| title | Vaticanol C, a resveratrol tetramer, activates PPARα and PPARβ/δ <it>in vitro </it>and <it>in vivo</it> |
| title_full | Vaticanol C, a resveratrol tetramer, activates PPARα and PPARβ/δ <it>in vitro </it>and <it>in vivo</it> |
| title_fullStr | Vaticanol C, a resveratrol tetramer, activates PPARα and PPARβ/δ <it>in vitro </it>and <it>in vivo</it> |
| title_full_unstemmed | Vaticanol C, a resveratrol tetramer, activates PPARα and PPARβ/δ <it>in vitro </it>and <it>in vivo</it> |
| title_short | Vaticanol C, a resveratrol tetramer, activates PPARα and PPARβ/δ <it>in vitro </it>and <it>in vivo</it> |
| title_sort | vaticanol c a resveratrol tetramer activates pparα and pparβ δ it in vitro it and it in vivo it |
| url | http://www.nutritionandmetabolism.com/content/7/1/46 |
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