Glycogen synthase kinase 3 (GSK-3) controls T-cell motility and interactions with antigen presenting cells
Abstract Objective The threonine/serine kinase glycogen synthase kinase 3 (GSK-3) targets multiple substrates in T-cells, regulating the expression of Tbet and PD-1 on T-cells. However, it has been unclear whether GSK-3 can affect the motility of T-cells and their interactions with antigen presentin...
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| Format: | Article |
| Language: | English |
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BMC
2020-03-01
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| Series: | BMC Research Notes |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s13104-020-04971-0 |
| _version_ | 1819230282172071936 |
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| author | Alison Taylor Christopher E. Rudd |
| author_facet | Alison Taylor Christopher E. Rudd |
| author_sort | Alison Taylor |
| collection | DOAJ |
| description | Abstract Objective The threonine/serine kinase glycogen synthase kinase 3 (GSK-3) targets multiple substrates in T-cells, regulating the expression of Tbet and PD-1 on T-cells. However, it has been unclear whether GSK-3 can affect the motility of T-cells and their interactions with antigen presenting cells. Results Here, we show that GSK-3 controls T-cell motility and interactions with other cells. Inhibition of GSK-3, using structurally distinct inhibitors, reduced T-cell motility in terms of distance and displacement. While SB415286 reduced the number of cell-cell contacts, the dwell times of cells that established contacts with other cells did not differ for T-cells treated with SB415286. Further, the increase in cytolytic T-cell (CTL) function in killing tumor targets was not affected by the inhibition of motility. This data shows that the inhibition of GSK-3 has differential effects on T-cell motility and CTL function where the negative effects on cell–cell interactions is overridden by the increased cytolytic potential of CTLs. |
| first_indexed | 2024-12-23T11:26:35Z |
| format | Article |
| id | doaj.art-3e67b0601bb34177ae3e5c4e12bf4d39 |
| institution | Directory Open Access Journal |
| issn | 1756-0500 |
| language | English |
| last_indexed | 2024-12-23T11:26:35Z |
| publishDate | 2020-03-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Research Notes |
| spelling | doaj.art-3e67b0601bb34177ae3e5c4e12bf4d392022-12-21T17:48:55ZengBMCBMC Research Notes1756-05002020-03-011311710.1186/s13104-020-04971-0Glycogen synthase kinase 3 (GSK-3) controls T-cell motility and interactions with antigen presenting cellsAlison Taylor0Christopher E. Rudd1Leeds Institute of Medical Research, School of Medicine, University of LeedsCell Signalling Section, Department of Pathology, University of CambridgeAbstract Objective The threonine/serine kinase glycogen synthase kinase 3 (GSK-3) targets multiple substrates in T-cells, regulating the expression of Tbet and PD-1 on T-cells. However, it has been unclear whether GSK-3 can affect the motility of T-cells and their interactions with antigen presenting cells. Results Here, we show that GSK-3 controls T-cell motility and interactions with other cells. Inhibition of GSK-3, using structurally distinct inhibitors, reduced T-cell motility in terms of distance and displacement. While SB415286 reduced the number of cell-cell contacts, the dwell times of cells that established contacts with other cells did not differ for T-cells treated with SB415286. Further, the increase in cytolytic T-cell (CTL) function in killing tumor targets was not affected by the inhibition of motility. This data shows that the inhibition of GSK-3 has differential effects on T-cell motility and CTL function where the negative effects on cell–cell interactions is overridden by the increased cytolytic potential of CTLs.http://link.springer.com/article/10.1186/s13104-020-04971-0T-cellsGSK-3MotilityCell contacts |
| spellingShingle | Alison Taylor Christopher E. Rudd Glycogen synthase kinase 3 (GSK-3) controls T-cell motility and interactions with antigen presenting cells BMC Research Notes T-cells GSK-3 Motility Cell contacts |
| title | Glycogen synthase kinase 3 (GSK-3) controls T-cell motility and interactions with antigen presenting cells |
| title_full | Glycogen synthase kinase 3 (GSK-3) controls T-cell motility and interactions with antigen presenting cells |
| title_fullStr | Glycogen synthase kinase 3 (GSK-3) controls T-cell motility and interactions with antigen presenting cells |
| title_full_unstemmed | Glycogen synthase kinase 3 (GSK-3) controls T-cell motility and interactions with antigen presenting cells |
| title_short | Glycogen synthase kinase 3 (GSK-3) controls T-cell motility and interactions with antigen presenting cells |
| title_sort | glycogen synthase kinase 3 gsk 3 controls t cell motility and interactions with antigen presenting cells |
| topic | T-cells GSK-3 Motility Cell contacts |
| url | http://link.springer.com/article/10.1186/s13104-020-04971-0 |
| work_keys_str_mv | AT alisontaylor glycogensynthasekinase3gsk3controlstcellmotilityandinteractionswithantigenpresentingcells AT christophererudd glycogensynthasekinase3gsk3controlstcellmotilityandinteractionswithantigenpresentingcells |