An improved method for the covalent attachment of glycolipids to solid supports and macromolecules.
A simplified method is presented for the oxidation of the olefinic bond of the sphingosine moiety of glycosphingolipids to a carboxyl group. Coupling of such “glycolipid acids” to glass beads, agarose gels, proteins, and polyacrylic hydrazide polymers is described. Solid supports and macromolecules...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
1979-02-01
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| Series: | Journal of Lipid Research |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227520406418 |
| _version_ | 1818386190693302272 |
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| author | W W Young, Jr R A Laine S Hakomori |
| author_facet | W W Young, Jr R A Laine S Hakomori |
| author_sort | W W Young, Jr |
| collection | DOAJ |
| description | A simplified method is presented for the oxidation of the olefinic bond of the sphingosine moiety of glycosphingolipids to a carboxyl group. Coupling of such “glycolipid acids” to glass beads, agarose gels, proteins, and polyacrylic hydrazide polymers is described. Solid supports and macromolecules that have been derivatized in this fashion are useful reagents for a variety of studies in cell biology and immunology. |
| first_indexed | 2024-12-14T03:50:07Z |
| format | Article |
| id | doaj.art-3e8a8b851b024f399e8e39d69fee4124 |
| institution | Directory Open Access Journal |
| issn | 0022-2275 |
| language | English |
| last_indexed | 2024-12-14T03:50:07Z |
| publishDate | 1979-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Lipid Research |
| spelling | doaj.art-3e8a8b851b024f399e8e39d69fee41242022-12-21T23:18:15ZengElsevierJournal of Lipid Research0022-22751979-02-01202275278An improved method for the covalent attachment of glycolipids to solid supports and macromolecules.W W Young, JrR A LaineS HakomoriA simplified method is presented for the oxidation of the olefinic bond of the sphingosine moiety of glycosphingolipids to a carboxyl group. Coupling of such “glycolipid acids” to glass beads, agarose gels, proteins, and polyacrylic hydrazide polymers is described. Solid supports and macromolecules that have been derivatized in this fashion are useful reagents for a variety of studies in cell biology and immunology.http://www.sciencedirect.com/science/article/pii/S0022227520406418 |
| spellingShingle | W W Young, Jr R A Laine S Hakomori An improved method for the covalent attachment of glycolipids to solid supports and macromolecules. Journal of Lipid Research |
| title | An improved method for the covalent attachment of glycolipids to solid supports and macromolecules. |
| title_full | An improved method for the covalent attachment of glycolipids to solid supports and macromolecules. |
| title_fullStr | An improved method for the covalent attachment of glycolipids to solid supports and macromolecules. |
| title_full_unstemmed | An improved method for the covalent attachment of glycolipids to solid supports and macromolecules. |
| title_short | An improved method for the covalent attachment of glycolipids to solid supports and macromolecules. |
| title_sort | improved method for the covalent attachment of glycolipids to solid supports and macromolecules |
| url | http://www.sciencedirect.com/science/article/pii/S0022227520406418 |
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