A Rapid, Multiplexed, High-Throughput Flow-Through Membrane Immunoassay: A Convenient Alternative to ELISA

A Rapid, Multiplexed, High-Throughput Flow-Through Membrane Immunoassay: A Convenient Alternative to ELISA

This paper describes a rapid, high-throughput flow-through membrane immunoassay (FMIA) platform. A nitrocellulose membrane was spotted in an array format with multiple capture and control reagents for each sample detection area, and assay steps were carried out by sequential aspiration of sample and...

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Bibliographic Details
Main Authors: Gonzalo J. Domingo, Paul Yager, Christiane Dolecek, Jeremy Farrar, Buddha Basnyat, Stephen G. Baker, Sabina Dongol, Amit Arjyal, Jennifer L. Osborn, Katherine G. McKenzie, Mitra Singhal, Sujatha Ramachandran, Barry Lutz
Format: Article
Language:English
Published: MDPI AG 2013-04-01
Series:Diagnostics
Subjects:
flow-through membrane immunoassay (FMIA)
enzyme-linked immunosorbent assay (ELISA)
multiplex
indirect IgM assay
Salmonella enterica serovar Typhi
typhoid
serodiagnosis
low resource setting
Online Access:http://www.mdpi.com/2075-4418/3/2/244

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http://www.mdpi.com/2075-4418/3/2/244

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