Enhancing the in vivo stability of polycation gene carriers by using PEGylated hyaluronic acid as a shielding system
To increase the in vivo stability of cationic gene carriers and avoid the adverse effects of their positive charge, we synthesized a new shielding material by conjugating low molecular weight polyethylene glycol (PEG) to a hyaluronic acid (HA) core. The HA-PEG conjugate assembled with the positively...
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| Format: | Article |
| Language: | English |
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Compuscript Ltd
2022-09-01
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| Series: | BIO Integration |
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| Online Access: | https://www.ingentaconnect.com/contentone/cscript/bioi/2022/00000003/00000003/art00002 |
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| author | Jiaxue Liu Xiaoli Bao Irina Kolesnik Boyan Jia Zihan Yu Caiyun Xing Jiawen Huang Tingting Gu Xiaotong Shao Alexey Kletskov Andreii S. Kritchenkov Vladimir Potkin Wenliang Li |
| author_facet | Jiaxue Liu Xiaoli Bao Irina Kolesnik Boyan Jia Zihan Yu Caiyun Xing Jiawen Huang Tingting Gu Xiaotong Shao Alexey Kletskov Andreii S. Kritchenkov Vladimir Potkin Wenliang Li |
| author_sort | Jiaxue Liu |
| collection | DOAJ |
| description | To increase the in vivo stability of cationic gene carriers and avoid the adverse effects of their positive charge, we synthesized a new shielding material by conjugating low molecular weight polyethylene glycol (PEG) to a hyaluronic acid (HA) core. The HA-PEG conjugate assembled with the positively charged complex, forming a protective layer through electrostatic interactions. DNA/polyetherimide/HA-PEG (DNA/PEI/HA-PEG) nanoparticles had higher stability than both DNA/polyethyleneimine (DNA/PEI) and DNA/PEI/HA complexes. Furthermore, DNA/PEI/HA-PEG nanoparticles also showed a diminished nonspecific response toward serum proteins in vivo. The in vivo transfection efficiency was also enhanced by the low cytotoxicity and the improved stability; therefore, this material might be promising for use in gene delivery applications. |
| first_indexed | 2024-04-11T11:34:23Z |
| format | Article |
| id | doaj.art-3ea4883ce124478ebc1116bb7b168da3 |
| institution | Directory Open Access Journal |
| issn | 2712-0082 |
| language | English |
| last_indexed | 2024-04-11T11:34:23Z |
| publishDate | 2022-09-01 |
| publisher | Compuscript Ltd |
| record_format | Article |
| series | BIO Integration |
| spelling | doaj.art-3ea4883ce124478ebc1116bb7b168da32022-12-22T04:26:01ZengCompuscript LtdBIO Integration2712-00822022-09-013310311110.15212/bioi-2021-0033Enhancing the in vivo stability of polycation gene carriers by using PEGylated hyaluronic acid as a shielding systemJiaxue Liu0Xiaoli Bao1Irina Kolesnik2Boyan Jia3Zihan Yu4Caiyun Xing5Jiawen Huang6Tingting Gu7Xiaotong Shao8Alexey Kletskov9Andreii S. Kritchenkov10Vladimir Potkin11Wenliang Li12Jilin Collaborative Innovation Center for Antibody Engineering, Jilin Medical University, Jilin, ChinaNorman Bethune Health Science Center, Jilin University, Jilin, ChinaInstitute of Physical Organic Chemistry of National Academy of Sciences of Belarus, 13 Surganov Str., 220072, Minsk, BelarusJilin Collaborative Innovation Center for Antibody Engineering, Jilin Medical University, Jilin, ChinaSchool of Pharmacy, Jilin Medical University, Jilin, ChinaSchool of Pharmacy, Jilin Medical University, Jilin, ChinaSchool of Pharmacy, Jilin Medical University, Jilin, ChinaSchool of Pharmacy, Jilin Medical University, Jilin, ChinaSchool of Medical Laboratory, Jilin Medical University, Jilin, ChinaPeoples’ Friendship University of Russia (RUDN University), Miklukho-Maklaya St. 6, Moscow, 117198, Russian FederationPeoples’ Friendship University of Russia (RUDN University), Miklukho-Maklaya St. 6, Moscow, 117198, Russian FederationInstitute of Physical Organic Chemistry of National Academy of Sciences of Belarus, 13 Surganov Str., 220072, Minsk, BelarusJilin Collaborative Innovation Center for Antibody Engineering, Jilin Medical University, Jilin, ChinaTo increase the in vivo stability of cationic gene carriers and avoid the adverse effects of their positive charge, we synthesized a new shielding material by conjugating low molecular weight polyethylene glycol (PEG) to a hyaluronic acid (HA) core. The HA-PEG conjugate assembled with the positively charged complex, forming a protective layer through electrostatic interactions. DNA/polyetherimide/HA-PEG (DNA/PEI/HA-PEG) nanoparticles had higher stability than both DNA/polyethyleneimine (DNA/PEI) and DNA/PEI/HA complexes. Furthermore, DNA/PEI/HA-PEG nanoparticles also showed a diminished nonspecific response toward serum proteins in vivo. The in vivo transfection efficiency was also enhanced by the low cytotoxicity and the improved stability; therefore, this material might be promising for use in gene delivery applications.https://www.ingentaconnect.com/contentone/cscript/bioi/2022/00000003/00000003/art00002peghyaluronic acidstabilitygene deliverycationic gene carriers |
| spellingShingle | Jiaxue Liu Xiaoli Bao Irina Kolesnik Boyan Jia Zihan Yu Caiyun Xing Jiawen Huang Tingting Gu Xiaotong Shao Alexey Kletskov Andreii S. Kritchenkov Vladimir Potkin Wenliang Li Enhancing the in vivo stability of polycation gene carriers by using PEGylated hyaluronic acid as a shielding system BIO Integration peg hyaluronic acid stability gene delivery cationic gene carriers |
| title | Enhancing the in vivo stability of polycation gene carriers by using PEGylated hyaluronic acid as a shielding system |
| title_full | Enhancing the in vivo stability of polycation gene carriers by using PEGylated hyaluronic acid as a shielding system |
| title_fullStr | Enhancing the in vivo stability of polycation gene carriers by using PEGylated hyaluronic acid as a shielding system |
| title_full_unstemmed | Enhancing the in vivo stability of polycation gene carriers by using PEGylated hyaluronic acid as a shielding system |
| title_short | Enhancing the in vivo stability of polycation gene carriers by using PEGylated hyaluronic acid as a shielding system |
| title_sort | enhancing the in vivo stability of polycation gene carriers by using pegylated hyaluronic acid as a shielding system |
| topic | peg hyaluronic acid stability gene delivery cationic gene carriers |
| url | https://www.ingentaconnect.com/contentone/cscript/bioi/2022/00000003/00000003/art00002 |
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