Generation of non-human primate CAR Tregs using artificial antigen-presenting cells, simian tropic lentiviral vectors, and antigen-specific restimulation

Generation of non-human primate CAR Tregs using artificial antigen-presenting cells, simian tropic lentiviral vectors, and antigen-specific restimulation

Summary: It is technically challenging to generate large doses of regulatory T cells (Tregs) engineered to express a chimeric antigen receptor (CAR) in non-human primates (NHP). Here, we have optimized the manufacturing of CAR Tregs by stringent sorting of Tregs, stimulation by artificial antigen-pr...

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Bibliographic Details
Main Authors: Gavin I. Ellis, Mosha Z. Deng, Delaine W. Winn, Kimberly E. Coker, Divanshu Shukla, Vijay Bhoj, Michael C. Milone, Raimon Duran-Struuck, James L. Riley
Format: Article
Language:English
Published: Elsevier 2022-12-01
Series:STAR Protocols
Subjects:
Cell culture
Cell isolation
Flow cytometry/Mass cytometry
Immunology
Model organisms
Tissue engineering
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166722006645

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http://www.sciencedirect.com/science/article/pii/S2666166722006645

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