Inhibition of chromatin condensation disrupts planar cell migration
Cell migration involves the actin cytoskeleton, and recently recognized nuclear involvement. In this study, we explore the impact of chromatin remodeling on cell migration using NIH 3T3 cells and a scratch wound assay subjected to pharmacological interventions. We inhibit histone deacetylases (HDACs...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2024-12-01
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| Series: | Nucleus |
| Subjects: | |
| Online Access: | https://www.tandfonline.com/doi/10.1080/19491034.2024.2325961 |
| _version_ | 1826945134050148352 |
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| author | Jack Forman Briar Hine Samantha Kaonis Soham Ghosh |
| author_facet | Jack Forman Briar Hine Samantha Kaonis Soham Ghosh |
| author_sort | Jack Forman |
| collection | DOAJ |
| description | Cell migration involves the actin cytoskeleton, and recently recognized nuclear involvement. In this study, we explore the impact of chromatin remodeling on cell migration using NIH 3T3 cells and a scratch wound assay subjected to pharmacological interventions. We inhibit histone deacetylases (HDACs) with Trichostatin A (TSA) and methyltransferase EZH2 with GSK126 to modulate chromatin compaction. Our results indicate that chromatin modifications impair wound closure efficiency, reduce individual cell migration speed, and disrupt migration persistence. Live-cell imaging reveals dynamic intranuclear chromatin remodeling and nuclear shape parameters during migration, influenced by both small- and large-scale chromatin remodeling. The altered nuclear shape is associated with disrupted cell and nuclear mechanics, suggesting a crucial interplay between chromatin remodeling, nuclear mechanics and migration. These findings shed light on the intricate connection between intranuclear chromatin dynamics, nuclear mechanics, and cell migration, providing a basis for further investigations into the molecular mechanisms governing these processes. |
| first_indexed | 2024-04-25T00:53:41Z |
| format | Article |
| id | doaj.art-3eda852a8d2d450eb1ebd8656a8bb10f |
| institution | Directory Open Access Journal |
| issn | 1949-1034 1949-1042 |
| language | English |
| last_indexed | 2025-02-17T20:49:58Z |
| publishDate | 2024-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Nucleus |
| spelling | doaj.art-3eda852a8d2d450eb1ebd8656a8bb10f2024-12-09T07:27:13ZengTaylor & Francis GroupNucleus1949-10341949-10422024-12-0115110.1080/19491034.2024.2325961Inhibition of chromatin condensation disrupts planar cell migrationJack Forman0Briar Hine1Samantha Kaonis2Soham Ghosh3School of Biomedical Engineering, Colorado State University, Fort Collins, CO, USASchool of Biomedical Engineering, Colorado State University, Fort Collins, CO, USASchool of Biomedical Engineering, Colorado State University, Fort Collins, CO, USASchool of Biomedical Engineering, Colorado State University, Fort Collins, CO, USACell migration involves the actin cytoskeleton, and recently recognized nuclear involvement. In this study, we explore the impact of chromatin remodeling on cell migration using NIH 3T3 cells and a scratch wound assay subjected to pharmacological interventions. We inhibit histone deacetylases (HDACs) with Trichostatin A (TSA) and methyltransferase EZH2 with GSK126 to modulate chromatin compaction. Our results indicate that chromatin modifications impair wound closure efficiency, reduce individual cell migration speed, and disrupt migration persistence. Live-cell imaging reveals dynamic intranuclear chromatin remodeling and nuclear shape parameters during migration, influenced by both small- and large-scale chromatin remodeling. The altered nuclear shape is associated with disrupted cell and nuclear mechanics, suggesting a crucial interplay between chromatin remodeling, nuclear mechanics and migration. These findings shed light on the intricate connection between intranuclear chromatin dynamics, nuclear mechanics, and cell migration, providing a basis for further investigations into the molecular mechanisms governing these processes.https://www.tandfonline.com/doi/10.1080/19491034.2024.2325961Cell migrationchromatinchromatin remodeling and compactionepigeneticsmechanobiology |
| spellingShingle | Jack Forman Briar Hine Samantha Kaonis Soham Ghosh Inhibition of chromatin condensation disrupts planar cell migration Nucleus Cell migration chromatin chromatin remodeling and compaction epigenetics mechanobiology |
| title | Inhibition of chromatin condensation disrupts planar cell migration |
| title_full | Inhibition of chromatin condensation disrupts planar cell migration |
| title_fullStr | Inhibition of chromatin condensation disrupts planar cell migration |
| title_full_unstemmed | Inhibition of chromatin condensation disrupts planar cell migration |
| title_short | Inhibition of chromatin condensation disrupts planar cell migration |
| title_sort | inhibition of chromatin condensation disrupts planar cell migration |
| topic | Cell migration chromatin chromatin remodeling and compaction epigenetics mechanobiology |
| url | https://www.tandfonline.com/doi/10.1080/19491034.2024.2325961 |
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