Effects of <i>Lonicera japonica</i> Flower Bud Extract on <i>Citrobacter rodentium</i>-Induced Digestive Tract Infection

<b>Background:</b> Although antibiotic therapy is currently a gold standard for bacterial infections, it is not used for severe diseases like enterohemorrhagic <i>Escherichia coli</i>, in which the Shiga toxin is overproduced by antibiotic action. The <i>Lonicera japoni...

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Main Authors: Masaaki Minami, Toshiaki Makino
Format: Article
Language:English
Published: MDPI AG 2020-08-01
Series:Medicines
Subjects:
Online Access:https://www.mdpi.com/2305-6320/7/9/52
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author Masaaki Minami
Toshiaki Makino
author_facet Masaaki Minami
Toshiaki Makino
author_sort Masaaki Minami
collection DOAJ
description <b>Background:</b> Although antibiotic therapy is currently a gold standard for bacterial infections, it is not used for severe diseases like enterohemorrhagic <i>Escherichia coli</i>, in which the Shiga toxin is overproduced by antibiotic action. The <i>Lonicera japonica</i> flower bud (LJF) is an herbal component used against purulent diseases in traditional Japanese and Chinese medicine. We investigated the effects of LJF extract (LJFE) on <i>Citrobacter rodentium</i>-induced digestive tract infection in a mouse model. <b>Methods:</b><i>Citrobacter rodentium</i> and LJFE were orally administered to C57BL/6 mice. The survival rate and bacterial colonization in the large intestine, mesenteric lymph node, and blood of mice were evaluated. Cytokines secreted from intraperitoneal macrophages of LJFE-treated mice were measured using ELISA. Moreover, the phagocytic activity of intraperitoneal macrophages against <i>Citrobacter rodentium</i> was compared between LJFE- or chlorogenic acid (CGA)-treated mice. <b>Results:</b> LJFE significantly increased the survival rate and decreased <i>Citrobacter rodentium</i> colonization in mice. Moreover, the values of tumor necrosis factor-α, interleukin-1β, and interferon-γ secreted from macrophages were increased following LJFE treatment. While macrophages of LJFE-treated mice showed a significant phagocytic activity, macrophages of CGA-treated mice only showed a phagocytic tendency. <b>Conclusions:</b> LJF may be useful for treating <i>Citrobacter rodentium</i>-induced digestive tract infection.
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spelling doaj.art-3f253e47638441b1a83c9608b320df502023-11-20T11:33:10ZengMDPI AGMedicines2305-63202020-08-01795210.3390/medicines7090052Effects of <i>Lonicera japonica</i> Flower Bud Extract on <i>Citrobacter rodentium</i>-Induced Digestive Tract InfectionMasaaki Minami0Toshiaki Makino1Department of Bacteriology, Graduate School of Medical Sciences, Nagoya City University, 1 Kawasumi, Mizuho-ku, Nagoya 467-8601, JapanDepartment of Pharmacognosy, Graduate School of Pharmaceutical Sciences, Nagoya City University, 3-1 Tanabe-Dori, Mizuho-ku, Nagoya 467-8603, Japan<b>Background:</b> Although antibiotic therapy is currently a gold standard for bacterial infections, it is not used for severe diseases like enterohemorrhagic <i>Escherichia coli</i>, in which the Shiga toxin is overproduced by antibiotic action. The <i>Lonicera japonica</i> flower bud (LJF) is an herbal component used against purulent diseases in traditional Japanese and Chinese medicine. We investigated the effects of LJF extract (LJFE) on <i>Citrobacter rodentium</i>-induced digestive tract infection in a mouse model. <b>Methods:</b><i>Citrobacter rodentium</i> and LJFE were orally administered to C57BL/6 mice. The survival rate and bacterial colonization in the large intestine, mesenteric lymph node, and blood of mice were evaluated. Cytokines secreted from intraperitoneal macrophages of LJFE-treated mice were measured using ELISA. Moreover, the phagocytic activity of intraperitoneal macrophages against <i>Citrobacter rodentium</i> was compared between LJFE- or chlorogenic acid (CGA)-treated mice. <b>Results:</b> LJFE significantly increased the survival rate and decreased <i>Citrobacter rodentium</i> colonization in mice. Moreover, the values of tumor necrosis factor-α, interleukin-1β, and interferon-γ secreted from macrophages were increased following LJFE treatment. While macrophages of LJFE-treated mice showed a significant phagocytic activity, macrophages of CGA-treated mice only showed a phagocytic tendency. <b>Conclusions:</b> LJF may be useful for treating <i>Citrobacter rodentium</i>-induced digestive tract infection.https://www.mdpi.com/2305-6320/7/9/52<i>Lonicera japonica</i><i>Citrobacter rodentium</i>bacterial digestive tract infectionchlorogenic acidintraperitoneal macrophages
spellingShingle Masaaki Minami
Toshiaki Makino
Effects of <i>Lonicera japonica</i> Flower Bud Extract on <i>Citrobacter rodentium</i>-Induced Digestive Tract Infection
Medicines
<i>Lonicera japonica</i>
<i>Citrobacter rodentium</i>
bacterial digestive tract infection
chlorogenic acid
intraperitoneal macrophages
title Effects of <i>Lonicera japonica</i> Flower Bud Extract on <i>Citrobacter rodentium</i>-Induced Digestive Tract Infection
title_full Effects of <i>Lonicera japonica</i> Flower Bud Extract on <i>Citrobacter rodentium</i>-Induced Digestive Tract Infection
title_fullStr Effects of <i>Lonicera japonica</i> Flower Bud Extract on <i>Citrobacter rodentium</i>-Induced Digestive Tract Infection
title_full_unstemmed Effects of <i>Lonicera japonica</i> Flower Bud Extract on <i>Citrobacter rodentium</i>-Induced Digestive Tract Infection
title_short Effects of <i>Lonicera japonica</i> Flower Bud Extract on <i>Citrobacter rodentium</i>-Induced Digestive Tract Infection
title_sort effects of i lonicera japonica i flower bud extract on i citrobacter rodentium i induced digestive tract infection
topic <i>Lonicera japonica</i>
<i>Citrobacter rodentium</i>
bacterial digestive tract infection
chlorogenic acid
intraperitoneal macrophages
url https://www.mdpi.com/2305-6320/7/9/52
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