Coupling between the Basic Replicon and the Kis-Kid Maintenance System of Plasmid R1: Modulation by Kis Antitoxin Levels and Involvement in Control of Plasmid Replication
kis-kid, the auxiliary maintenance system of plasmid R1 and copB, the auxiliary copy number control gene of this plasmid, contribute to increase plasmid replication efficiency in cells with lower than average copy number. It is thought that Kis antitoxin levels decrease in these cells and that this...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2015-02-01
|
| Series: | Toxins |
| Subjects: | |
| Online Access: | http://www.mdpi.com/2072-6651/7/2/478 |
| _version_ | 1817992988107735040 |
|---|---|
| author | Juan López-Villarejo Damián Lobato-Márquez Ramón Díaz-Orejas |
| author_facet | Juan López-Villarejo Damián Lobato-Márquez Ramón Díaz-Orejas |
| author_sort | Juan López-Villarejo |
| collection | DOAJ |
| description | kis-kid, the auxiliary maintenance system of plasmid R1 and copB, the auxiliary copy number control gene of this plasmid, contribute to increase plasmid replication efficiency in cells with lower than average copy number. It is thought that Kis antitoxin levels decrease in these cells and that this acts as the switch that activates the Kid toxin; activated Kid toxin reduces copB-mRNA levels and this increases RepA levels that increases plasmid copy number. In support of this model we now report that: (i) the Kis antitoxin levels do decrease in cells containing a mini-R1 plasmid carrying a repA mutation that reduces plasmid copy number; (ii) kid-dependent replication rescue is abolished in cells in which the Kis antitoxin levels or the CopB levels are increased. Unexpectedly we found that this coordination significantly increases both the copy number of the repA mutant and of the wt mini-R1 plasmid. This indicates that the coordination between plasmid replication functions and kis-kid system contributes significantly to control plasmid R1 replication. |
| first_indexed | 2024-04-14T01:33:46Z |
| format | Article |
| id | doaj.art-3f3137faff334e44bcdf02d50d21317a |
| institution | Directory Open Access Journal |
| issn | 2072-6651 |
| language | English |
| last_indexed | 2024-04-14T01:33:46Z |
| publishDate | 2015-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Toxins |
| spelling | doaj.art-3f3137faff334e44bcdf02d50d21317a2022-12-22T02:20:03ZengMDPI AGToxins2072-66512015-02-017247849210.3390/toxins7020478toxins7020478Coupling between the Basic Replicon and the Kis-Kid Maintenance System of Plasmid R1: Modulation by Kis Antitoxin Levels and Involvement in Control of Plasmid ReplicationJuan López-Villarejo0Damián Lobato-Márquez1Ramón Díaz-Orejas2Department of Molecular Microbiology and Infection Biology, C/ Ramiro de Maéztu 9, Centro de Investigaciones Biológicas-CSIC, 28040 Madrid, SpainDepartment of Molecular Microbiology and Infection Biology, C/ Ramiro de Maéztu 9, Centro de Investigaciones Biológicas-CSIC, 28040 Madrid, SpainDepartment of Molecular Microbiology and Infection Biology, C/ Ramiro de Maéztu 9, Centro de Investigaciones Biológicas-CSIC, 28040 Madrid, Spainkis-kid, the auxiliary maintenance system of plasmid R1 and copB, the auxiliary copy number control gene of this plasmid, contribute to increase plasmid replication efficiency in cells with lower than average copy number. It is thought that Kis antitoxin levels decrease in these cells and that this acts as the switch that activates the Kid toxin; activated Kid toxin reduces copB-mRNA levels and this increases RepA levels that increases plasmid copy number. In support of this model we now report that: (i) the Kis antitoxin levels do decrease in cells containing a mini-R1 plasmid carrying a repA mutation that reduces plasmid copy number; (ii) kid-dependent replication rescue is abolished in cells in which the Kis antitoxin levels or the CopB levels are increased. Unexpectedly we found that this coordination significantly increases both the copy number of the repA mutant and of the wt mini-R1 plasmid. This indicates that the coordination between plasmid replication functions and kis-kid system contributes significantly to control plasmid R1 replication.http://www.mdpi.com/2072-6651/7/2/478plasmid R1plasmid replication and copy number controlparD (kis-kid) antitoxin-toxin systemKis antitoxinClpAP proteasetranscriptional regulationCopB repressorcoupling plasmid maintenance systems |
| spellingShingle | Juan López-Villarejo Damián Lobato-Márquez Ramón Díaz-Orejas Coupling between the Basic Replicon and the Kis-Kid Maintenance System of Plasmid R1: Modulation by Kis Antitoxin Levels and Involvement in Control of Plasmid Replication Toxins plasmid R1 plasmid replication and copy number control parD (kis-kid) antitoxin-toxin system Kis antitoxin ClpAP protease transcriptional regulation CopB repressor coupling plasmid maintenance systems |
| title | Coupling between the Basic Replicon and the Kis-Kid Maintenance System of Plasmid R1: Modulation by Kis Antitoxin Levels and Involvement in Control of Plasmid Replication |
| title_full | Coupling between the Basic Replicon and the Kis-Kid Maintenance System of Plasmid R1: Modulation by Kis Antitoxin Levels and Involvement in Control of Plasmid Replication |
| title_fullStr | Coupling between the Basic Replicon and the Kis-Kid Maintenance System of Plasmid R1: Modulation by Kis Antitoxin Levels and Involvement in Control of Plasmid Replication |
| title_full_unstemmed | Coupling between the Basic Replicon and the Kis-Kid Maintenance System of Plasmid R1: Modulation by Kis Antitoxin Levels and Involvement in Control of Plasmid Replication |
| title_short | Coupling between the Basic Replicon and the Kis-Kid Maintenance System of Plasmid R1: Modulation by Kis Antitoxin Levels and Involvement in Control of Plasmid Replication |
| title_sort | coupling between the basic replicon and the kis kid maintenance system of plasmid r1 modulation by kis antitoxin levels and involvement in control of plasmid replication |
| topic | plasmid R1 plasmid replication and copy number control parD (kis-kid) antitoxin-toxin system Kis antitoxin ClpAP protease transcriptional regulation CopB repressor coupling plasmid maintenance systems |
| url | http://www.mdpi.com/2072-6651/7/2/478 |
| work_keys_str_mv | AT juanlopezvillarejo couplingbetweenthebasicrepliconandthekiskidmaintenancesystemofplasmidr1modulationbykisantitoxinlevelsandinvolvementincontrolofplasmidreplication AT damianlobatomarquez couplingbetweenthebasicrepliconandthekiskidmaintenancesystemofplasmidr1modulationbykisantitoxinlevelsandinvolvementincontrolofplasmidreplication AT ramondiazorejas couplingbetweenthebasicrepliconandthekiskidmaintenancesystemofplasmidr1modulationbykisantitoxinlevelsandinvolvementincontrolofplasmidreplication |