Tyrosine nitration of a humanized anti-cocaine mAb differentially affects ligand binding of cocaine and its metabolites

Tyrosine nitration of a humanized anti-cocaine mAb differentially affects ligand binding of cocaine and its metabolites

Tetranitromethane was used to selectively modify tyrosine residues of a humanized anti-cocaine mAb (h2E2), under development for the treatment of cocaine use disorders. The effect of mild tyrosine nitration on the affinity of cocaine and two high affinity cocaine metabolites, cocaethylene and benzoy...

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Bibliographic Details
Main Authors: Terence L. Kirley, Kenneth D. Greis, Andrew B. Norman
Format: Article
Language:English
Published: Elsevier 2022-07-01
Series:Biochemistry and Biophysics Reports
Subjects:
Monoclonal antibody
Cocaine binding
Tyrosine nitration
DASPMI rotor dye
Differential scanning fluorimetry
Ligand affinity
Online Access:http://www.sciencedirect.com/science/article/pii/S2405580822000784

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http://www.sciencedirect.com/science/article/pii/S2405580822000784

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