Assessment of the Immunomodulatory Properties of the Probiotic Strain <i>Lactobacillus paracasei</i> K5 In Vitro and In Vivo
<i>Lactobacillus paracasei</i> K5 is a lactic acid bacteria (LAB) strain that has been isolated from dairy products. Previous studies have established its probiotic potential in a series of in vitro tests, including molecular characterization, safety profiling, and tolerability of the ga...
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MDPI AG
2020-05-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/8/5/709 |
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| author | Pelagia Chondrou Athanasios Karapetsas Despoina Eugenia Kiousi Stavros Vasileiadis Petros Ypsilantis Sotiris Botaitis Athanasios Alexopoulos Stavros Plessas Eugenia Bezirtzoglou Alex Galanis |
| author_facet | Pelagia Chondrou Athanasios Karapetsas Despoina Eugenia Kiousi Stavros Vasileiadis Petros Ypsilantis Sotiris Botaitis Athanasios Alexopoulos Stavros Plessas Eugenia Bezirtzoglou Alex Galanis |
| author_sort | Pelagia Chondrou |
| collection | DOAJ |
| description | <i>Lactobacillus paracasei</i> K5 is a lactic acid bacteria (LAB) strain that has been isolated from dairy products. Previous studies have established its probiotic potential in a series of in vitro tests, including molecular characterization, safety profiling, and tolerability of the gastrointestinal tract conditions. To characterize its beneficial actions on the host, we have shown previously that <i>L. paracasei</i> K5 adheres to Caco-2 cells and exerts anti-proliferative effects through the induction of apoptosis. In the present study, we focused on the immunomodulatory potential of this strain. We employed the dorsal-air-pouch mouse model of inflammation and recorded an eight-fold increase in the recruitment of immune cells in mice treated with the probiotic strain, compared to the control group. Analysis of the exudates revealed significant changes in the expression of pro-inflammatory mediators on site. Treatment of Caco-2 cells with <i>L. paracasei</i> K5 induced significant upregulation of cytokines interleukin-1α (IL-1α), ΙL-1β, IL-6, tumor necrosis factor-alpha (TNF-α), the chemokine C-X-C motif ligand 2 (CXCL2), and the inflammation markers soluble intercellular adhesion molecule (sICAM) and metallopeptidase inhibitor-1 (TIMP-1). Transient induction of the Toll-like receptors (TLRs) 2, 4, 6, and 9 expression levels was recorded by real-time PCR analysis. These results highlight the immunomodulatory potential of this strain and further support its probiotic character. |
| first_indexed | 2024-03-10T19:54:21Z |
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| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-10T19:54:21Z |
| publishDate | 2020-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-3f5922f909ae4b6eab67690ecc0c3f3f2023-11-20T00:03:49ZengMDPI AGMicroorganisms2076-26072020-05-018570910.3390/microorganisms8050709Assessment of the Immunomodulatory Properties of the Probiotic Strain <i>Lactobacillus paracasei</i> K5 In Vitro and In VivoPelagia Chondrou0Athanasios Karapetsas1Despoina Eugenia Kiousi2Stavros Vasileiadis3Petros Ypsilantis4Sotiris Botaitis5Athanasios Alexopoulos6Stavros Plessas7Eugenia Bezirtzoglou8Alex Galanis9Department of Molecular Biology and Genetics, Faculty of Health Sciences, Democritus University of Thrace, Alexandroupolis 68100, GreeceDepartment of Molecular Biology and Genetics, Faculty of Health Sciences, Democritus University of Thrace, Alexandroupolis 68100, GreeceDepartment of Molecular Biology and Genetics, Faculty of Health Sciences, Democritus University of Thrace, Alexandroupolis 68100, GreeceLaboratory of Experimental Surgery and Surgical Research, Medical School, Faculty of Health Sciences, Democritus University of Thrace, Alexandroupolis 68100, GreeceLaboratory of Experimental Surgery and Surgical Research, Medical School, Faculty of Health Sciences, Democritus University of Thrace, Alexandroupolis 68100, GreeceLaboratory of Experimental Surgery and Surgical Research, Medical School, Faculty of Health Sciences, Democritus University of Thrace, Alexandroupolis 68100, GreeceLaboratory of Microbiology, Biotechnology and Hygiene, Faculty of Agricultural Development, Democritus University of Thrace, Orestiada 68200, GreeceLaboratory of Microbiology, Biotechnology and Hygiene, Faculty of Agricultural Development, Democritus University of Thrace, Orestiada 68200, GreeceLaboratory of Hygiene and Environmental Protection, Medical School, Faculty of Health Sciences, Democritus University of Thrace, Alexandroupolis 68100, GreeceDepartment of Molecular Biology and Genetics, Faculty of Health Sciences, Democritus University of Thrace, Alexandroupolis 68100, Greece<i>Lactobacillus paracasei</i> K5 is a lactic acid bacteria (LAB) strain that has been isolated from dairy products. Previous studies have established its probiotic potential in a series of in vitro tests, including molecular characterization, safety profiling, and tolerability of the gastrointestinal tract conditions. To characterize its beneficial actions on the host, we have shown previously that <i>L. paracasei</i> K5 adheres to Caco-2 cells and exerts anti-proliferative effects through the induction of apoptosis. In the present study, we focused on the immunomodulatory potential of this strain. We employed the dorsal-air-pouch mouse model of inflammation and recorded an eight-fold increase in the recruitment of immune cells in mice treated with the probiotic strain, compared to the control group. Analysis of the exudates revealed significant changes in the expression of pro-inflammatory mediators on site. Treatment of Caco-2 cells with <i>L. paracasei</i> K5 induced significant upregulation of cytokines interleukin-1α (IL-1α), ΙL-1β, IL-6, tumor necrosis factor-alpha (TNF-α), the chemokine C-X-C motif ligand 2 (CXCL2), and the inflammation markers soluble intercellular adhesion molecule (sICAM) and metallopeptidase inhibitor-1 (TIMP-1). Transient induction of the Toll-like receptors (TLRs) 2, 4, 6, and 9 expression levels was recorded by real-time PCR analysis. These results highlight the immunomodulatory potential of this strain and further support its probiotic character.https://www.mdpi.com/2076-2607/8/5/709probioticscytokinesair pouchimmunomodulationToll-like receptors |
| spellingShingle | Pelagia Chondrou Athanasios Karapetsas Despoina Eugenia Kiousi Stavros Vasileiadis Petros Ypsilantis Sotiris Botaitis Athanasios Alexopoulos Stavros Plessas Eugenia Bezirtzoglou Alex Galanis Assessment of the Immunomodulatory Properties of the Probiotic Strain <i>Lactobacillus paracasei</i> K5 In Vitro and In Vivo Microorganisms probiotics cytokines air pouch immunomodulation Toll-like receptors |
| title | Assessment of the Immunomodulatory Properties of the Probiotic Strain <i>Lactobacillus paracasei</i> K5 In Vitro and In Vivo |
| title_full | Assessment of the Immunomodulatory Properties of the Probiotic Strain <i>Lactobacillus paracasei</i> K5 In Vitro and In Vivo |
| title_fullStr | Assessment of the Immunomodulatory Properties of the Probiotic Strain <i>Lactobacillus paracasei</i> K5 In Vitro and In Vivo |
| title_full_unstemmed | Assessment of the Immunomodulatory Properties of the Probiotic Strain <i>Lactobacillus paracasei</i> K5 In Vitro and In Vivo |
| title_short | Assessment of the Immunomodulatory Properties of the Probiotic Strain <i>Lactobacillus paracasei</i> K5 In Vitro and In Vivo |
| title_sort | assessment of the immunomodulatory properties of the probiotic strain i lactobacillus paracasei i k5 in vitro and in vivo |
| topic | probiotics cytokines air pouch immunomodulation Toll-like receptors |
| url | https://www.mdpi.com/2076-2607/8/5/709 |
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