High-Throughput Analysis of T-DNA Location and Structure Using Sequence Capture.

Agrobacterium-mediated transformation of plants with T-DNA is used both to introduce transgenes and for mutagenesis. Conventional approaches used to identify the genomic location and the structure of the inserted T-DNA are laborious and high-throughput methods using next-generation sequencing are be...

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Bibliographic Details
Main Authors: Soichi Inagaki, Isabelle M Henry, Meric C Lieberman, Luca Comai
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2015-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC4596565?pdf=render