Salivary β-glucosidase as a direct factor influencing the occurrence of halitosis
β-Glucosidases are enzymes present in all living organisms, playing a pivotal role in diverse biological processes. These enzymes cleave β-glycosidic bonds between carbohydrates, or between a carbohydrate and a non-carbohydrate moiety, which may result in the liberation of volatile aglycones. Releas...
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| Format: | Article |
| Language: | English |
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Elsevier
2021-07-01
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| Series: | Biochemistry and Biophysics Reports |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405580821000595 |
| _version_ | 1819097791593447424 |
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| author | Lucimari Teixeira Essenfelder Anderson Albino Gomes Jefferson Luis Meirelles Coimbra Marcelo Alves Moreira Sandra Maria Ferraz David José Miquelluti Gustavo Felippe da Silva Maria de Lourdes Borba Magalhães |
| author_facet | Lucimari Teixeira Essenfelder Anderson Albino Gomes Jefferson Luis Meirelles Coimbra Marcelo Alves Moreira Sandra Maria Ferraz David José Miquelluti Gustavo Felippe da Silva Maria de Lourdes Borba Magalhães |
| author_sort | Lucimari Teixeira Essenfelder |
| collection | DOAJ |
| description | β-Glucosidases are enzymes present in all living organisms, playing a pivotal role in diverse biological processes. These enzymes cleave β-glycosidic bonds between carbohydrates, or between a carbohydrate and a non-carbohydrate moiety, which may result in the liberation of volatile aglycones. Released compounds execute diverse physiological roles, while the industry takes advantage of exogenously added β-glucosidases for aroma enrichment during food and beverage production. β-Glucosidase enzymatic activity has been reported in human saliva and given the fact that these enzymes are involved in aroma release, we investigated here the correlation between β-glucosidase activity in human saliva and the occurrence of halitosis. Measurement of salivary enzyme activity of 48 volunteers was performed using p-nitrophenyl-β-d-glucopyranoside as substrate. Each volunteer was clinically evaluated by a dental surgeon and clinical and laboratorial data were statistically analyzed. Gas-chromatography of saliva headspace allowed the analysis of the direct role of exogenous β-glucosidase on aromatic /volatile profile of saliva samples. The data demonstrated a positive correlation between halitosis and enzymatic activity, suggesting that the enzyme exerts a direct role in the occurrence of bad breath. Gas-chromatography analysis demonstrated that exogenously added enzyme led to the alteration of volatile organic content, confirming a direct contribution of β-glucosidase activity on saliva volatile compounds release. Although halitosis is a multifactorial condition, the complete understanding of all governing factors may allow the development of more effective treatment strategies. Such studies may pave the way to the use of β-glucosidase inhibitors for halitosis clinical management. |
| first_indexed | 2024-12-22T00:20:42Z |
| format | Article |
| id | doaj.art-3fb0e9b7049040b0ae907e666d5e150a |
| institution | Directory Open Access Journal |
| issn | 2405-5808 |
| language | English |
| last_indexed | 2024-12-22T00:20:42Z |
| publishDate | 2021-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Biochemistry and Biophysics Reports |
| spelling | doaj.art-3fb0e9b7049040b0ae907e666d5e150a2022-12-21T18:45:10ZengElsevierBiochemistry and Biophysics Reports2405-58082021-07-0126100965Salivary β-glucosidase as a direct factor influencing the occurrence of halitosisLucimari Teixeira Essenfelder0Anderson Albino Gomes1Jefferson Luis Meirelles Coimbra2Marcelo Alves Moreira3Sandra Maria Ferraz4David José Miquelluti5Gustavo Felippe da Silva6Maria de Lourdes Borba Magalhães7Biochemistry Laboratory, Center of Agroveterinary Sciences, State University of Santa Catarina, Lages, Santa Catarina, 88520-000, BrazilBiochemistry Laboratory, Center of Agroveterinary Sciences, State University of Santa Catarina, Lages, Santa Catarina, 88520-000, BrazilDepartment of Soil and Natural Resources, Center of Agroveterinary Sciences, State University of Santa Catarina, Lages, Santa Catarina, 88520-000, BrazilDepartment of Soil and Natural Resources, Center of Agroveterinary Sciences, State University of Santa Catarina, Lages, Santa Catarina, 88520-000, BrazilDepartment of Veterinary Medicine, Center of Agroveterinary Sciences, State University of Santa Catarina, Lages, Santa Catarina, 88520-000, BrazilDepartment of Soil and Natural Resources, Center of Agroveterinary Sciences, State University of Santa Catarina, Lages, Santa Catarina, 88520-000, BrazilBiochemistry Laboratory, Center of Agroveterinary Sciences, State University of Santa Catarina, Lages, Santa Catarina, 88520-000, BrazilBiochemistry Laboratory, Center of Agroveterinary Sciences, State University of Santa Catarina, Lages, Santa Catarina, 88520-000, Brazil; Corresponding author. Luiz de Camões 2090, Lages, SC, Brazil.β-Glucosidases are enzymes present in all living organisms, playing a pivotal role in diverse biological processes. These enzymes cleave β-glycosidic bonds between carbohydrates, or between a carbohydrate and a non-carbohydrate moiety, which may result in the liberation of volatile aglycones. Released compounds execute diverse physiological roles, while the industry takes advantage of exogenously added β-glucosidases for aroma enrichment during food and beverage production. β-Glucosidase enzymatic activity has been reported in human saliva and given the fact that these enzymes are involved in aroma release, we investigated here the correlation between β-glucosidase activity in human saliva and the occurrence of halitosis. Measurement of salivary enzyme activity of 48 volunteers was performed using p-nitrophenyl-β-d-glucopyranoside as substrate. Each volunteer was clinically evaluated by a dental surgeon and clinical and laboratorial data were statistically analyzed. Gas-chromatography of saliva headspace allowed the analysis of the direct role of exogenous β-glucosidase on aromatic /volatile profile of saliva samples. The data demonstrated a positive correlation between halitosis and enzymatic activity, suggesting that the enzyme exerts a direct role in the occurrence of bad breath. Gas-chromatography analysis demonstrated that exogenously added enzyme led to the alteration of volatile organic content, confirming a direct contribution of β-glucosidase activity on saliva volatile compounds release. Although halitosis is a multifactorial condition, the complete understanding of all governing factors may allow the development of more effective treatment strategies. Such studies may pave the way to the use of β-glucosidase inhibitors for halitosis clinical management.http://www.sciencedirect.com/science/article/pii/S2405580821000595BiofilmHalitosisOral healthSalivaβ-Glucosidase |
| spellingShingle | Lucimari Teixeira Essenfelder Anderson Albino Gomes Jefferson Luis Meirelles Coimbra Marcelo Alves Moreira Sandra Maria Ferraz David José Miquelluti Gustavo Felippe da Silva Maria de Lourdes Borba Magalhães Salivary β-glucosidase as a direct factor influencing the occurrence of halitosis Biochemistry and Biophysics Reports Biofilm Halitosis Oral health Saliva β-Glucosidase |
| title | Salivary β-glucosidase as a direct factor influencing the occurrence of halitosis |
| title_full | Salivary β-glucosidase as a direct factor influencing the occurrence of halitosis |
| title_fullStr | Salivary β-glucosidase as a direct factor influencing the occurrence of halitosis |
| title_full_unstemmed | Salivary β-glucosidase as a direct factor influencing the occurrence of halitosis |
| title_short | Salivary β-glucosidase as a direct factor influencing the occurrence of halitosis |
| title_sort | salivary β glucosidase as a direct factor influencing the occurrence of halitosis |
| topic | Biofilm Halitosis Oral health Saliva β-Glucosidase |
| url | http://www.sciencedirect.com/science/article/pii/S2405580821000595 |
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