Determination of 22 Free Amino Acids in Food for Special Medical Purposes by Ultra-performance Liquid Chromatography
A method was established to determine 22 free amino acids in food for special medical purposes by ultra-performance liquid chromatography. After extraction with sulfosalicylic acid and derivation with 2,4-dinitrofluorobenzene (DNFB), the samples were eluted with acetonitrile water-ammonium formate a...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | zho |
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The editorial department of Science and Technology of Food Industry
2023-07-01
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| Series: | Shipin gongye ke-ji |
| Subjects: | |
| Online Access: | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2022080065 |
| _version_ | 1797737835277582336 |
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| author | Lili YIN Hong ZHENG Zhi CHENG Lanxiang LU Xia XUE Jun WANG Mei HU Yanming LIU |
| author_facet | Lili YIN Hong ZHENG Zhi CHENG Lanxiang LU Xia XUE Jun WANG Mei HU Yanming LIU |
| author_sort | Lili YIN |
| collection | DOAJ |
| description | A method was established to determine 22 free amino acids in food for special medical purposes by ultra-performance liquid chromatography. After extraction with sulfosalicylic acid and derivation with 2,4-dinitrofluorobenzene (DNFB), the samples were eluted with acetonitrile water-ammonium formate as mobile phase. Amino acids were separated by HSS T3 column and detected with diode-array detector by external standard method. Results showed that, the 22 amino acids were linear in the range of 2~100 µg/mL with correlation coefficients higher than 0.99. The limits of detection (S/N≥3) were 10 mg/100 g, and the limits of quantification (S/N≥10) were 25 mg/100 g. The recoveries were in the range of 90.1% and 109.1% with relative standard deviation (RSD) from 0.8% to 6.9%. This method was simple, efficient, accurate and reproducible. It was suitable for determination of 22 free amino acids in food for special medical purposes, which could provide technical support to ensure the quality and safety. |
| first_indexed | 2024-03-12T13:34:13Z |
| format | Article |
| id | doaj.art-3fb6c7fe18b54fbe9c6a8cea590f43b1 |
| institution | Directory Open Access Journal |
| issn | 1002-0306 |
| language | zho |
| last_indexed | 2024-03-12T13:34:13Z |
| publishDate | 2023-07-01 |
| publisher | The editorial department of Science and Technology of Food Industry |
| record_format | Article |
| series | Shipin gongye ke-ji |
| spelling | doaj.art-3fb6c7fe18b54fbe9c6a8cea590f43b12023-08-24T06:00:30ZzhoThe editorial department of Science and Technology of Food IndustryShipin gongye ke-ji1002-03062023-07-01441426427110.13386/j.issn1002-0306.20220800652022080065-14Determination of 22 Free Amino Acids in Food for Special Medical Purposes by Ultra-performance Liquid ChromatographyLili YIN0Hong ZHENG1Zhi CHENG2Lanxiang LU3Xia XUE4Jun WANG5Mei HU6Yanming LIU7Shandong Institute for Food and Drug Control, Shandong Research Center of Engineering and Technology for Quality Control of Food for Special Medical Purposes, Shandong Research Center of Engineering and Technology for Safety Inspection of Food and Drug, Jinan 250101, ChinaShandong Institute for Food and Drug Control, Shandong Research Center of Engineering and Technology for Quality Control of Food for Special Medical Purposes, Shandong Research Center of Engineering and Technology for Safety Inspection of Food and Drug, Jinan 250101, ChinaShandong Institute for Food and Drug Control, Shandong Research Center of Engineering and Technology for Quality Control of Food for Special Medical Purposes, Shandong Research Center of Engineering and Technology for Safety Inspection of Food and Drug, Jinan 250101, ChinaShandong Institute for Food and Drug Control, Shandong Research Center of Engineering and Technology for Quality Control of Food for Special Medical Purposes, Shandong Research Center of Engineering and Technology for Safety Inspection of Food and Drug, Jinan 250101, ChinaShandong Institute for Food and Drug Control, Shandong Research Center of Engineering and Technology for Quality Control of Food for Special Medical Purposes, Shandong Research Center of Engineering and Technology for Safety Inspection of Food and Drug, Jinan 250101, ChinaShandong Institute for Food and Drug Control, Shandong Research Center of Engineering and Technology for Quality Control of Food for Special Medical Purposes, Shandong Research Center of Engineering and Technology for Safety Inspection of Food and Drug, Jinan 250101, ChinaShandong Institute for Food and Drug Control, Shandong Research Center of Engineering and Technology for Quality Control of Food for Special Medical Purposes, Shandong Research Center of Engineering and Technology for Safety Inspection of Food and Drug, Jinan 250101, ChinaShandong Institute for Food and Drug Control, Shandong Research Center of Engineering and Technology for Quality Control of Food for Special Medical Purposes, Shandong Research Center of Engineering and Technology for Safety Inspection of Food and Drug, Jinan 250101, ChinaA method was established to determine 22 free amino acids in food for special medical purposes by ultra-performance liquid chromatography. After extraction with sulfosalicylic acid and derivation with 2,4-dinitrofluorobenzene (DNFB), the samples were eluted with acetonitrile water-ammonium formate as mobile phase. Amino acids were separated by HSS T3 column and detected with diode-array detector by external standard method. Results showed that, the 22 amino acids were linear in the range of 2~100 µg/mL with correlation coefficients higher than 0.99. The limits of detection (S/N≥3) were 10 mg/100 g, and the limits of quantification (S/N≥10) were 25 mg/100 g. The recoveries were in the range of 90.1% and 109.1% with relative standard deviation (RSD) from 0.8% to 6.9%. This method was simple, efficient, accurate and reproducible. It was suitable for determination of 22 free amino acids in food for special medical purposes, which could provide technical support to ensure the quality and safety.http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2022080065food for special medical purposesfree amino acidpre-column derivatization2,4-dinitrofluorobenzeneultra-performance liquid chromatography |
| spellingShingle | Lili YIN Hong ZHENG Zhi CHENG Lanxiang LU Xia XUE Jun WANG Mei HU Yanming LIU Determination of 22 Free Amino Acids in Food for Special Medical Purposes by Ultra-performance Liquid Chromatography Shipin gongye ke-ji food for special medical purposes free amino acid pre-column derivatization 2,4-dinitrofluorobenzene ultra-performance liquid chromatography |
| title | Determination of 22 Free Amino Acids in Food for Special Medical Purposes by Ultra-performance Liquid Chromatography |
| title_full | Determination of 22 Free Amino Acids in Food for Special Medical Purposes by Ultra-performance Liquid Chromatography |
| title_fullStr | Determination of 22 Free Amino Acids in Food for Special Medical Purposes by Ultra-performance Liquid Chromatography |
| title_full_unstemmed | Determination of 22 Free Amino Acids in Food for Special Medical Purposes by Ultra-performance Liquid Chromatography |
| title_short | Determination of 22 Free Amino Acids in Food for Special Medical Purposes by Ultra-performance Liquid Chromatography |
| title_sort | determination of 22 free amino acids in food for special medical purposes by ultra performance liquid chromatography |
| topic | food for special medical purposes free amino acid pre-column derivatization 2,4-dinitrofluorobenzene ultra-performance liquid chromatography |
| url | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2022080065 |
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