THE QUANTIFICATION OF SOME BIOACTIVE COMPOUNDS IN THE FRUITS OF FOUR BLACKBERRY (Rubus fruticosus L.) CULTIVARS, PROPAGATED BY TISSUE CULTURE
The goal of the research was the quantitative determination of biochemical compounds (ascorbic acid, reducing sugars, tannins and titratable acidity) in Rubus fruticosus L. fruits – blackberries ('Melana', 'Triple Crown', 'Arapaho' and 'Thornfree'), propagated...
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| Format: | Article |
| Language: | English |
| Published: |
Alexandru Ioan Cuza University of Iasi
2022-12-01
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| Series: | Journal of Plant Development |
| Subjects: | |
| Online Access: | https://plant-journal.uaic.ro/docs/2022/4.pdf |
| _version_ | 1797962127511650304 |
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| author | Ion ROSCA Aliona GLIJIN Nina CIORCHINA Maria TABARA Alina CUTCOVSCHI-MUSTUC Tudor RALEA Nina ZDIORUC Alexandru MÎRZA |
| author_facet | Ion ROSCA Aliona GLIJIN Nina CIORCHINA Maria TABARA Alina CUTCOVSCHI-MUSTUC Tudor RALEA Nina ZDIORUC Alexandru MÎRZA |
| author_sort | Ion ROSCA |
| collection | DOAJ |
| description | The goal of the research was the quantitative determination of biochemical compounds (ascorbic acid, reducing sugars, tannins and titratable acidity) in Rubus fruticosus L. fruits – blackberries ('Melana', 'Triple Crown', 'Arapaho' and 'Thornfree'), propagated by tissue culture and cultivated under the ecological conditions of the Republic of Moldova. The spectrophotometric determination of ascorbic acid revealed that the highest content of this phytocompound was quantified in the 'Arapaho' blackberries, 48.28 mg/100 g, followed by the 'Thornfree' cultivar (41.69 mg/100 g), which is an amount about twice as high as in the 'Melana' blackberries (23.8 mg/100 g). The high amount of vitamin C in the researched blackberries makes them comparable to raspberries and gooseberries. The content of reducing sugars varied between 4.72% and 7.26%. The 'Triple Crown' cultivar was characterized by the maximum amounts of these biochemical compounds, and the lowest amounts were found in the fruits of the 'Thornfree' blackberry cultivar. The evaluation of the titratable acidity showed that the highest index of this parameter was recorded in the 'Melana' blackberries (0.74% / 0.77% as compared with malic / citric acid. The quantification of the tannins revealed that the fruits of the 'Triple Crown' cultivar have the lowest tannin content (1.97%), the amount being more than twice lower than the maximum amount (4.16%), recorded in the blackberries of the 'Thornfree' cultivar. The results of the study led to the conclusion that the fruits of Rubus fruticosus L., obtained from plants which had been micropropagated in the Embryology and Biotechnology Laboratory (NBGI) and grown under the conditions of the Republic of Moldova are a good source of biologically active substances and can be proposed for commercial production as a promising organic food product. |
| first_indexed | 2024-04-11T01:08:25Z |
| format | Article |
| id | doaj.art-3fb9405e55cf4094b029153aafad83d4 |
| institution | Directory Open Access Journal |
| issn | 2065-3158 2066-9917 |
| language | English |
| last_indexed | 2024-04-11T01:08:25Z |
| publishDate | 2022-12-01 |
| publisher | Alexandru Ioan Cuza University of Iasi |
| record_format | Article |
| series | Journal of Plant Development |
| spelling | doaj.art-3fb9405e55cf4094b029153aafad83d42023-01-04T09:28:39ZengAlexandru Ioan Cuza University of IasiJournal of Plant Development2065-31582066-99172022-12-0129556810.47743/jpd.2022.29.1.908THE QUANTIFICATION OF SOME BIOACTIVE COMPOUNDS IN THE FRUITS OF FOUR BLACKBERRY (Rubus fruticosus L.) CULTIVARS, PROPAGATED BY TISSUE CULTUREIon ROSCA0https://orcid.org/0000-0002-1304-8033Aliona GLIJIN1Nina CIORCHINA2Maria TABARA3Alina CUTCOVSCHI-MUSTUC4Tudor RALEA5Nina ZDIORUC6Alexandru MÎRZA7“Alexandru Ciubotaru” National Botanical Garden (Institute) – Chisinau, Republic of Moldova“Alexandru Ciubotaru” National Botanical Garden (Institute) – Chisinau, Republic of Moldova“Alexandru Ciubotaru” National Botanical Garden (Institute) – Chisinau, Republic of Moldova“Alexandru Ciubotaru” National Botanical Garden (Institute) – Chisinau, Republic of Moldova“Alexandru Ciubotaru” National Botanical Garden (Institute) – Chisinau, Republic of MoldovaInstitute of Genetics, Physiology and Plant Protection – Chisinau, Republic of MoldovaInstitute of Genetics, Physiology and Plant Protection – Chisinau, Republic of Moldova“Alexandru Ciubotaru” National Botanical Garden (Institute) – Chisinau, Republic of MoldovaThe goal of the research was the quantitative determination of biochemical compounds (ascorbic acid, reducing sugars, tannins and titratable acidity) in Rubus fruticosus L. fruits – blackberries ('Melana', 'Triple Crown', 'Arapaho' and 'Thornfree'), propagated by tissue culture and cultivated under the ecological conditions of the Republic of Moldova. The spectrophotometric determination of ascorbic acid revealed that the highest content of this phytocompound was quantified in the 'Arapaho' blackberries, 48.28 mg/100 g, followed by the 'Thornfree' cultivar (41.69 mg/100 g), which is an amount about twice as high as in the 'Melana' blackberries (23.8 mg/100 g). The high amount of vitamin C in the researched blackberries makes them comparable to raspberries and gooseberries. The content of reducing sugars varied between 4.72% and 7.26%. The 'Triple Crown' cultivar was characterized by the maximum amounts of these biochemical compounds, and the lowest amounts were found in the fruits of the 'Thornfree' blackberry cultivar. The evaluation of the titratable acidity showed that the highest index of this parameter was recorded in the 'Melana' blackberries (0.74% / 0.77% as compared with malic / citric acid. The quantification of the tannins revealed that the fruits of the 'Triple Crown' cultivar have the lowest tannin content (1.97%), the amount being more than twice lower than the maximum amount (4.16%), recorded in the blackberries of the 'Thornfree' cultivar. The results of the study led to the conclusion that the fruits of Rubus fruticosus L., obtained from plants which had been micropropagated in the Embryology and Biotechnology Laboratory (NBGI) and grown under the conditions of the Republic of Moldova are a good source of biologically active substances and can be proposed for commercial production as a promising organic food product.https://plant-journal.uaic.ro/docs/2022/4.pdf'arapaho'ascorbic acidbiochemical parametersblackberry'melana'tannins'thornfree'titratable acidity'triple crown'reducing sugars |
| spellingShingle | Ion ROSCA Aliona GLIJIN Nina CIORCHINA Maria TABARA Alina CUTCOVSCHI-MUSTUC Tudor RALEA Nina ZDIORUC Alexandru MÎRZA THE QUANTIFICATION OF SOME BIOACTIVE COMPOUNDS IN THE FRUITS OF FOUR BLACKBERRY (Rubus fruticosus L.) CULTIVARS, PROPAGATED BY TISSUE CULTURE Journal of Plant Development 'arapaho' ascorbic acid biochemical parameters blackberry 'melana' tannins 'thornfree' titratable acidity 'triple crown' reducing sugars |
| title | THE QUANTIFICATION OF SOME BIOACTIVE COMPOUNDS IN THE FRUITS OF FOUR BLACKBERRY (Rubus fruticosus L.) CULTIVARS, PROPAGATED BY TISSUE CULTURE |
| title_full | THE QUANTIFICATION OF SOME BIOACTIVE COMPOUNDS IN THE FRUITS OF FOUR BLACKBERRY (Rubus fruticosus L.) CULTIVARS, PROPAGATED BY TISSUE CULTURE |
| title_fullStr | THE QUANTIFICATION OF SOME BIOACTIVE COMPOUNDS IN THE FRUITS OF FOUR BLACKBERRY (Rubus fruticosus L.) CULTIVARS, PROPAGATED BY TISSUE CULTURE |
| title_full_unstemmed | THE QUANTIFICATION OF SOME BIOACTIVE COMPOUNDS IN THE FRUITS OF FOUR BLACKBERRY (Rubus fruticosus L.) CULTIVARS, PROPAGATED BY TISSUE CULTURE |
| title_short | THE QUANTIFICATION OF SOME BIOACTIVE COMPOUNDS IN THE FRUITS OF FOUR BLACKBERRY (Rubus fruticosus L.) CULTIVARS, PROPAGATED BY TISSUE CULTURE |
| title_sort | quantification of some bioactive compounds in the fruits of four blackberry rubus fruticosus l cultivars propagated by tissue culture |
| topic | 'arapaho' ascorbic acid biochemical parameters blackberry 'melana' tannins 'thornfree' titratable acidity 'triple crown' reducing sugars |
| url | https://plant-journal.uaic.ro/docs/2022/4.pdf |
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