A HaloTag-TEV genetic cassette for mechanical phenotyping of proteins from tissues

A HaloTag-TEV genetic cassette for mechanical phenotyping of proteins from tissues

Testing mechanical forces on native molecules in natural environments remains a challenge. Here the authors engineer titin to carry a HaloTag-TEV insertion to allow analysis of dynamics under force in muscle fibers.

Bibliographic Details
Main Authors: Jaime Andrés Rivas-Pardo, Yong Li, Zsolt Mártonfalvi, Rafael Tapia-Rojo, Andreas Unger, Ángel Fernández-Trasancos, Elías Herrero-Galán, Diana Velázquez-Carreras, Julio M. Fernández, Wolfgang A. Linke, Jorge Alegre-Cebollada
Format: Article
Language:English
Published: Nature Portfolio 2020-04-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-020-15465-9

Internet

https://doi.org/10.1038/s41467-020-15465-9

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