A HaloTag-TEV genetic cassette for mechanical phenotyping of proteins from tissues
Testing mechanical forces on native molecules in natural environments remains a challenge. Here the authors engineer titin to carry a HaloTag-TEV insertion to allow analysis of dynamics under force in muscle fibers.
Bibliographic Details
| Main Authors: |
Jaime Andrés Rivas-Pardo,
Yong Li,
Zsolt Mártonfalvi,
Rafael Tapia-Rojo,
Andreas Unger,
Ángel Fernández-Trasancos,
Elías Herrero-Galán,
Diana Velázquez-Carreras,
Julio M. Fernández,
Wolfgang A. Linke,
Jorge Alegre-Cebollada |
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| Format: | Article
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| Language: | English |
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| Published: |
Nature Portfolio
2020-04-01
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| Series: | Nature Communications
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| Online Access: | https://doi.org/10.1038/s41467-020-15465-9
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