Comparison of rRNA-based reverse transcription PCR and rDNA-based PCR for the detection of streptococci in root canal infections

Abstract Objective The rDNA-based method is unable to distinguish between alive and dead cells. Alternatively, bacterial viability can be assessed by molecular methods based on ribosomal RNA (rRNA). Therefore, this study aimed to detect viable streptococci in root canal samples using rRNA-based re...

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Bibliographic Details
Main Authors: Laís Cunha PRADO, Giulio GAVINI, Amanda da Costa SILVEIRA, Vitor Cesar NAKAMURA, Marcia Pinto Alves MAYER, Ericka Tavares PINHEIRO
Format: Article
Language:English
Published: University of São Paulo
Series:Journal of Applied Oral Science
Subjects:
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-77572019000100462&lng=en&tlng=en